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| Status |
Public on Oct 01, 2017 |
| Title |
RNA-Seq of hematopoietic stem cells from vehicle and estrogen-treated mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Purpose: The goals of this study were to determine genes in HSCs that are regulated by estrogen.
Methods: mRNA profiles of vehicle and estrogen treated wild-type (WT) and Esr1fl/fl;Mx1-Cre (ERa-KO) HSCs were generated by deep sequencing using Illumina HiSeq 2000 and Ion Torrent Proton platforms. The sequence reads that passed quality filters were analyzed at the transcript isoform level using DE-Seq2. qRT–PCR validation was performed using SYBR Green assays
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| Overall design |
mRNA profiles of vehicle and estrogen treated wild-type (WT) and Esr1fl/fl;Mx1-Cre (ERa-KO) HSCs were generated by deep sequencing using Ion Torrent platform
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| |
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| Contributor(s) |
Nakada D, Chapple R, Hoegenauer KA |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
May 21, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
Daisuke Nakada |
| E-mail(s) |
nakada@bcm.edu
|
| Organization name |
Baylor College of Medicine
|
| Department |
Molecular and Human Genetics
|
| Street address |
One Baylor Plaza
|
| City |
Houston |
| State/province |
Texas |
| ZIP/Postal code |
77030 |
| Country |
USA |
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| Platforms (1) |
| GPL18635 |
Ion Torrent Proton (Mus musculus) |
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| Samples (15)
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| Relations |
| BioProject |
PRJNA387370 |
| SRA |
SRP107690 |
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