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Series GSE9915 Query DataSets for GSE9915
Status Public on Nov 01, 2008
Title Transcript profiling of Lr1- and Lr34-mediated leaf rust resistance in wheat
Organism Triticum aestivum
Experiment type Expression profiling by array
Summary The wheat gene Lr34 confers partial resistance to all races of Puccinia triticina, the causal agent of wheat leaf rust. However, the biological basis for the exceptional durability of Lr34 is unclear. The Affymetrix wheat genome array was used to identify wheat genes differentially expressed in a compatible interaction (Tc), an R-gene mediated incompatible interaction (Tc-Lr1), and a race non-specific resistance interaction (Tc-Lr34) in response to infection challenge by P. triticina race 1 at anthesis. Transcriptome interrogation was conducted by comparing mock- and P. triticina-inoculated leaves harvested at 3 and 7 days post inoculation (dpi).
Keywords: Time course

 
Overall design The Affymetrix wheat genome array was used to identify wheat genes differentially expressed in a compatible interaction (Tc), an R-gene mediated incompatible interaction (Tc-Lr1), and a race non-specific resistance interaction (Tc-Lr34) in response to infection challenge by P. triticina race 1 at anthesis. Transcriptome interrogation was conducted on leaves harvested at 3 and 7 days post inoculation (dpi). The study utilized a randomized complete block design with three replicates for each genotype, and employed univariate analysis (t-tests) between mock- and P. triticina-inoculated plants within each genotype at each timepoint, for a total of six comparisons across the entire experiment, utilizing a total 36 Affymetrix Wheat Genome Array GeneChips.
 
Contributor(s) Bolton MD, Kolmer JA, Xu WW, Garvin DF
Citation(s) 18986248
Submission date Dec 17, 2007
Last update date Dec 04, 2018
Contact name David Garvin
E-mail(s) David.Garvin@ars.usda.gov
Organization name USDA
Department Plant Science Research Unit
Street address 411 Borlaug Hall, 1991 Upper Buford Circle
City St. Paul
State/province MN
ZIP/Postal code 55108
Country USA
 
Platforms (1)
GPL3802 [wheat] Affymetrix Wheat Genome Array
Samples (36)
GSM250883 Tc_3dpi_mock_rep1
GSM250884 Tc_3dpi_inoculated_rep1
GSM250885 TcLr1_3dpi_mock_rep1
Relations
BioProject PRJNA103909

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE9915_RAW.tar 286.4 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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