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| Status |
Public on Jul 30, 2017 |
| Title |
Expression data from rat endosalpinx at 3 hours after mating |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by array
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| Summary |
In the mammalian oviduct, mating regulates sperm storage, egg transport, fertilization, early embryonic development and estradiol metabolism. However, the main molecules responsible for these processes are poorly understood.
Herein, we used Affymetrix microarrays to conduct a transcriptomic analysis of the endosalpinx at 3 h after mating and used Qiagen´s Ingenuity Pathway Analysis to perform network modeling to identify the main mating-activated cellular processes in the endosalpinx and the possible upstream regulators of the mating-induced transcriptional response.
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| Overall design |
Females in pro-estrus were kept isolated (unmated rats) or were caged with fertile males at 2200 h of pro-estrus. Thirty minutes after caging with males, the presence of spermatozoa in the vaginal smear or a vaginal plug was verified. Females with spermatozoa or a vaginal plug were designated mated rats and remained caged with the males until 0130 h of estrus, 3 h after mating.
Under our environmental and experimental conditions, at 3 h after mating, sperm would have arrived in the oviduct, and ovulation would have occurred approximately 5 h later. Unmated and mated rats were sacrificed at 0130 h of estrus or 3 h after mating. Then, the endosalpinx was isolated from the oviducts and stored in TRIZOL reagent until processing.
We used six chips to hybridize six individual samples; 3 of them were samples from unmated rats, and 3 were samples from mated rats (N = 3 per group). We follow the manufacturer’s instructions.
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| Contributor(s) |
Zúñiga LM, Croxatto HB, Gutiérrez RA |
| Citation missing |
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| Submission date |
May 29, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
Lidia M. Zuniga |
| E-mail(s) |
lidia.zuniga@uantof.cl
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| Phone |
56995259092
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| Organization name |
Universidad de Antofagasta
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| Department |
Instituto Antofagasta
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| Lab |
Biología de la Reproducción
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| Street address |
Av. Universidad de Antofagasta 02800
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| City |
Antofagasta |
| State/province |
Antofagasta |
| ZIP/Postal code |
1240000 |
| Country |
Chile |
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| Platforms (1) |
| GPL6247 |
[RaGene-1_0-st] Affymetrix Rat Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (6)
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| GSM2643279 |
Endosalpinx-rat55, unmated biological rep1 |
| GSM2643280 |
Endosalpinx-rat61, unmated biological rep2 |
| GSM2643281 |
Endosalpinx-rat67, unmated biological rep3 |
| GSM2643282 |
Endosalpinx-rat44, mated biological rep1 |
| GSM2643283 |
Endosalpinx-rat43, mated biological rep2 |
| GSM2643284 |
Endosalpinx-rat53, mated biological rep3 |
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| Relations |
| BioProject |
PRJNA388320 |
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