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Series GSE99683 Query DataSets for GSE99683
Status Public on Jun 20, 2018
Title γδ T cells orchestrate the induction of protective type 2 immunity in neonatal influenza infection
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Influenza infection causes high rates of hospitalization and mortality in infants. γδ T cells are critical for immune responses against pathogens as regulators and effectors, especially in infants, and yet the roles of neonatal γδ T cells in influenza remain to be investigated. Here we report that γδ T cells were protective against mortality associated with neonatal influenza infection. Infection induced the accumulation and activation of γδ T cells, which transiently expressed IL-17a to enhance early IL-33 production by lung epithelial cells via STAT3 phosphorylation. Subsequently, this led to type 2 immune responses with elicited infiltration of ILC2s and Tregs resulting in increased amphiregulin secretion and tissue repair. Loss of γδ T cells did not alter viral clearance or IFN-γ production. Thus, our results identify a specific requirement for γδ T cells in influenza-infected neonates by initiating type 2 immune responses, mediating tissue homeostasis, and promoting lung integrity.
 
Overall design RNA-Seq of total RNA from whole-lung homogenates of influenza-infected wild-type and TCRδ-/- neonatal mice at 8 days post infection.
 
Contributor(s) Guo X, Crawford JC, Thomas PG
Citation(s) 30170813
NIH grant(s)
Grant ID Grant title Affiliation Name
R01 AI121832 A protective role for gamma delta T cells in respiratory infection ST. JUDE CHILDREN'S RESEARCH HOSPITAL PAUL G. THOMAS
Submission date Jun 05, 2017
Last update date Jul 25, 2021
Contact name Jeremy Chase Crawford
E-mail(s) jeremy.crawford@stjude.org
Organization name St. Jude Children's Research Hospital
Department Department of Host-Microbe Interactions
Street address MS 221, 262 Danny Thomas Place
City Memphis
State/province TN
ZIP/Postal code 38105-3678
Country USA
 
Platforms (1)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
Samples (6)
GSM2650132 D10TDWT-1
GSM2650133 D10TDWT-2
GSM2650134 D10TDWT-3
Relations
BioProject PRJNA389295
SRA SRP108621

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Supplementary file Size Download File type/resource
GSE99683_RAW.tar 1.1 Mb (http)(custom) TAR (of TAB)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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