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| Status |
Public on Dec 01, 2017 |
| Title |
Effects of WCE herbal extract on hormone-refractory PC-3 tumors |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Purpose: Androgen-deprivation therapy is the standard treatment for prostate cancer but fails in hormone-refractory prostate cancer. The anti-inflammatory plant Wedelia chinensis is rich in luteolin, apigenin, and wedelolactone that act synergistically to suppress androgen receptor activity in prostate cancer. Here, we evaluated the systemic antitumor effects of a standardized and effect-optimized Wedelia chinensis herbal extract (WCE) on hormone-refractory prostate cancer.
Methods: Hormone-refractory PC-3 orthotopic tumor mouse models were orally administered with WCE. The tumor transcriptomes were studied using RNA sequencing and Ingenuity Pathway Analysis to identify the molecular mechanisms.
Results: WCE significantly attenuated tumor growth and metastasis in orthotopic PC-3 xenografts. Transcriptomic analysis of genome-wide gene expression in the tumors revealed that WCE suppressed the expression of HIF1α, IKKα/β phosphorylation, and the downstream cytokines/chemokines.
Conclusions: A standardized preparation of Wedelia chinensis improved prostate cancer therapy through repressing NFκB-mediated cytokine expression in tumor cells and modulating the inflammatory tumor microenvironment. These data suggest that WCE functions through immunomodulation and has potential application as an adjuvant agent for the treatment of castration-resistant prostate cancer.
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| Overall design |
mRNA profiles of tumor xengrafts were generated by sequencing in 3 individual tumor samples from mice with vehicle treatment and 3 individual tumor samples from WCE-treated mice SRA Study accession: SRP108551; BioProject accession: PRJNA389147
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| |
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| Contributor(s) |
Tsai C, Hsiao P |
| Citation(s) |
29142311 |
| BioProject |
PRJNA389147 |
| |
| Submission date |
Jun 08, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Chin-Hsien Tsai |
| E-mail(s) |
tch@gate.sinica.edu.tw
|
| Organization name |
Academia Sinica
|
| Department |
ABRC
|
| Street address |
Nan-Kang
|
| City |
Taipei |
| State/province |
Others |
| ZIP/Postal code |
11529 |
| Country |
Taiwan |
| |
|
| Platforms (1) |
| GPL15456 |
Illumina HiScanSQ (Homo sapiens) |
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| Samples (6)
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| Relations |
| SRA |
SRP108551 |
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