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| Status |
Public on Aug 06, 2019 |
| Title |
Gene expression profile of primary epidermal keratinocytes (HEKs) stimulated with lawsone |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
The study aimed to interrogate whether the 1,4-naphtoquinone derivative lawsone activates the aryl hydrocarbon receptor (AhR) downstream transcriptional program in keratinocytes and is capable of modulating skin homeostasis. The study has been performed on human primary epidermal keratinocytes (HEKs), which represent the first line of defense against exogenous molecules. HEK cells were treated with the vehicle control DMSO (control), stimulated with lawsone or with the TLR2 ligand Pam2CSK4. Transcriptional profiles of HEK cells were studied to investigate the regulation of AhR -related genes, Nrf2-related genes and epidermal differentiation and keratin genes. We demonstrated that lawsone was sensed by keratinocytes and activated AhR. In particular, lawsone efficiently activated the transcriptional program of AhR and promoted keratinocyte differentiation.
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| Overall design |
Three-condition experiment: HEK cells treated with the vehicle control DMSO (control) vs. HEK cells stimulated with lawsone (10 µM) (Law). As negative control we used HEK cells stimulated with the TLR2 ligand Pam2CSK4 (300 ng/mL) (TLR2). One biological replicate. control= ctrl; lawsone= Law; Pam2CSK4=TLR2.
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| Contributor(s) |
Domaszewska T, Weiner 3rd J, Mollenkopf HJ, Lozza L |
| Citation(s) |
31350436 |
| |
| Submission date |
Jun 11, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
Teresa Domaszewska |
| E-mail(s) |
domaszewska@mpiib-berlin.mpg.de
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| Organization name |
Max Planck Institute for Infection Biology
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| Department |
Immunology
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| Street address |
Chariteplatz 1
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| City |
Berlin |
| State/province |
Berlin |
| ZIP/Postal code |
10117 |
| Country |
Germany |
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| Platforms (1) |
| GPL23572 |
Agilent-048908 8x60K whole genome incl V1-V2 linc BioVacSafe final 048908 [Probe Name version] |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA389993 |
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