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Series GSE9998 Query DataSets for GSE9998
Status Public on Dec 19, 2008
Title 14028 Promoter libraries through human PC3 tumor in nude mice
Platform organism Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
Sample organism Salmonella enterica subsp. enterica serovar Typhimurium
Experiment type Other
Summary Salmonella typhimurium 14028s promoter library (180,000 clones, both stable and destabilized GFP versions) recovered from either spleens / PC3 tumors 2 days after IV / IT delivery into nude mice, sorted for green bacteria using FACS, compared to the initial promoter library grown in LB Ampicillin broth, O/N at 37°C with aeration
Keywords: promoter screen
 
Overall design used 5 mice each for IT delivery of the 14028 promoter libraries into tumor or IV delivery and subsequent harvest from spleen. Sorted for green bacteria using FACS. Compared various sortings after these environments to the original promoter library after plasmid recovery and Klenow labeling of PCR amplified plasmid inserts by hybridization onto 380k custom-made Nimblegen Salmonella tiling oligo array
 
Contributor(s) Arrach N, Porwollik S, McClelland M
Citation(s) 18559530
Submission date Dec 20, 2007
Last update date Mar 17, 2012
Contact name Michael McClelland
E-mail(s) mcclelland.michael@gmail.com
Phone 858-336-9554
Organization name University of California, Irvine
Department Microbiology & Molecular Genetics
Street address 132 Med Surge I
City Irvine
State/province CA
ZIP/Postal code 92697
Country USA
 
Platforms (1)
GPL5728 NIMBLE_MMCCSAL01_380k
Samples (4)
GSM252718 14028_Promoterlibmix_stable+unstable_sort2_mouseSpleen(+)_115218_1
GSM252719 14028_Promoterlibmix_stable+unstable_sort1_mouseTumor(+)(-)(+)_115249_2
GSM252720 14028_Promoterlibmix_stable+unstable_sort2_mouseTumor(+)(-)(+)_115249_1
Relations
BioProject PRJNA104021

Download family Format
SOFT formatted family file(s) SOFTHelp
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Supplementary data files not provided
Processed data included within Sample table

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