|
| Status |
Public on Sep 06, 2007 |
| Title |
S.cerevisiae Ste12::13myc chIP chip replicate 1 (Slide 42415) |
| Sample type |
genomic |
| |
|
| Channel 1 |
| Source name |
Ste12::13myc
|
| Organism |
Saccharomyces cerevisiae |
| Characteristics |
Sigma1278b diploid strain (825/6 L1) containing Ste12 with C-terminal 13myc tag
|
| Biomaterial provider |
Anthony Borneman
|
| Growth protocol |
Grown at 30¡C to OD600 = 0.3 in SD media. Transferred to liquid SLAD for 4 hrs at 30¡C.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Chromatin immunoprecipitation (Borneman et al Genes Dev 2006)
|
| Label |
Cy5
|
| Label protocol |
Klenow random priming (Borneman et al Genes Dev 2006)
|
| |
|
| Channel 2 |
| Source name |
825/6 L1
|
| Organism |
Saccharomyces cerevisiae |
| Characteristics |
Sigma1278b diploid control strain
|
| Biomaterial provider |
Anthony Borneman
|
| Growth protocol |
Grown at 30¡C to OD600 = 0.3 in SD media. Transferred to liquid SLAD for 4 hrs at 30¡C.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Chromatin immunoprecipitation (Borneman et al Genes Dev 2006)
|
| Label |
Cy3
|
| Label protocol |
Klenow random priming (Borneman et al Genes Dev 2006)
|
| |
|
| |
| Hybridization protocol |
Nimblegen
|
| Scan protocol |
Nimblegen
|
| Description |
S.cerevisiae Ste12::13myc chIP chip replicate 1
|
| Data processing |
Samples were scanned and with each channel (635 (ch1) and 532 (ch2)) normalized separately according to Nimblegen protocols. log(2) ratios were calculated from these normalized 635 and 532 signals, with Random control spots removed during processing.
|
| |
|
| Submission date |
Mar 13, 2006 |
| Last update date |
Sep 06, 2007 |
| Contact name |
Anthony R Borneman |
| E-mail(s) |
anthony.borneman@awri.com.au
|
| Phone |
61 8 8303 6600
|
| Organization name |
The Australian Wine Research Institute
|
| Street address |
Paratoo Rd
|
| City |
Urrbrae |
| State/province |
South Australia |
| ZIP/Postal code |
5064 |
| Country |
Australia |
| |
|
| Platform ID |
GPL3541 |
| Series (2) |
| GSE4450 |
A comparison of high density oligonucleotide and PCR-based array platforms for chIP chip in yeast |
| GSE5421 |
Divergence of Transcription Factor Binding Sites in Related Yeast Species |
|
