|
| Status |
Public on Sep 07, 2013 |
| Title |
ZF_tumor_rep3 |
| Sample type |
RNA |
| |
|
| Source name |
Eye tumor
|
| Organism |
Danio rerio |
| Characteristics |
tissue: eye tumor
age: 4 months
|
| Treatment protocol |
No other treatments were applied
|
| Growth protocol |
Transgenic zebrafish expressing oncogenic smoa1 developed gross eye tumors and tumors from 4-month-old fish were dissected
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNAs were extracted with Trizol and then purified with Qiagen RNeasy MinElute Cleanup Kit
|
| Label |
Cy3
|
| Label protocol |
200 ng total RNA was labeled by using an Agilent Low Input Quick Amp labeling kit (part number 5190-2305) and following the one color protocol (v6.5)
|
| |
|
| Hybridization protocol |
Hybridization was performed at 65°C for 17 hours at 10 rpm in Agilent's hybridization chambers
|
| Scan protocol |
Arrays were scanned with an Agilent scanner (G2565CA) at 3 μm resolution
|
| Description |
Eye tumor, replicate 3
|
| Data processing |
Data were extracted by Agilent Feature Extraction software (v.10.5.1.1) using GE1_105_Jan09 protocol; and quantile normalization on background-subtracted signal intensity was performed among the samples
|
| |
|
| Submission date |
Sep 10, 2012 |
| Last update date |
Sep 07, 2013 |
| Contact name |
Yong-Dong Wang |
| E-mail(s) |
michael.wang@stjude.org
|
| Phone |
9015954224
|
| Organization name |
St. Jude Children's Research Hospital
|
| Street address |
262 Danny Thomas Pl, MS1135
|
| City |
Memphis |
| State/province |
TN |
| ZIP/Postal code |
38134 |
| Country |
USA |
| |
|
| Platform ID |
GPL14664 |
| Series (1) |
| GSE40761 |
Upregulated Hedgehog Signaling Promotes Zebrafish CNS Tumorigenesis |
|
