|
Status |
Public on Aug 05, 2013 |
Title |
Pulmonary fibroblasts_scleroderma-associated interstitial lung disease_ biological rep6 |
Sample type |
RNA |
|
|
Source name |
Primary pulmonary fibroblasts from scleroderma-associated interstitial lung disease patient 6
|
Organism |
Homo sapiens |
Characteristics |
tissue: Pulmonary fibroblasts disease status: Scleroderma associated interstitial lung disease
|
Treatment protocol |
Prior to RNA extraction confluent cultures were serum-deprived for 42 hours (media changed at 18 hours) in 0.1% bovine serum albumin.
|
Growth protocol |
Primary fibroblasts were cultured from lung biopsies as previously described (Abraham, D.J., et al. J.Biol.Chem 2000;275:15220-25).
|
Extracted molecule |
total RNA |
Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
Label |
Biotin
|
Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
|
|
Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Homo sapiens U133A1. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
Scan protocol |
GeneChips were scanned using the Affymetrix 30007G scanner.
|
Description |
Gene expression data from pulmonary fibroblasts in individuals with scleroderma-associated interstitial lung disease.
|
Data processing |
The data were analyzed with dCHIP using the invariant set normalisation method.
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|
|
Submission date |
Sep 12, 2012 |
Last update date |
Sep 01, 2016 |
Contact name |
Carmel Stock |
E-mail(s) |
c.stock@imperial.ac.uk
|
Organization name |
National Heart and Lung Institute
|
Department |
Interstitial Lung Disease
|
Street address |
1b Manresa Road
|
City |
London |
ZIP/Postal code |
SW3 6LR |
Country |
United Kingdom |
|
|
Platform ID |
GPL96 |
Series (1) |
GSE40839 |
Expression data from fibroblasts cultured from normal and fibrotic human lung tissue |
|
Relations |
Reanalyzed by |
GSE86363 |