|
| Status |
Public on Nov 02, 2012 |
| Title |
CLUSTER-NULL_ZT4 |
| Sample type |
RNA |
| |
|
| Source name |
CLUSTER-NULL_ZT4
|
| Organism |
Drosophila melanogaster |
| Characteristics |
tissue: Fly heads
genotype: w1118 ; mir959-962-KO
|
| Treatment protocol |
Flies were collected at specified time point and frozen on dry ice, heads were removed
|
| Growth protocol |
Adult flies were kept in constant 12 hr:12 hr light:dark conditions for 3 days before collection
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from fly heads using TRIzol reagent (Invitrogen) as suggested by the supplier
|
| Label |
biotin
|
| Label protocol |
RNA was labeled for hybridization to Drosophila Genome 2.0 arrays (Affymetrix) using the IVT Express Kit (Affymetrix).
|
| |
|
| Hybridization protocol |
RNA was hybrized to Drosophila Genome 2.0 arrays following manufacturers recommendations. Arrays were washed and stained on a Fluidics 450 Station running protocol Midi_euk2 v3.
|
| Scan protocol |
Drosophila Genome 2.0 arrays were scanned using a Affymetrix GeneChip Scanner 3000 and GCOS version 1.4.
|
| Data processing |
Normalized log2 values were computed from the CEL files using the GC-RMA algorithm in the Bioconductor analysis package for R software (http://www.r-project.org)
|
| |
|
| Submission date |
Sep 14, 2012 |
| Last update date |
Nov 02, 2012 |
| Contact name |
Stefan Pescatore |
| E-mail(s) |
spescato@brandeis.edu
|
| Phone |
781-736-3161
|
| Fax |
781-736-3164
|
| Organization name |
Brandeis University / HHMI
|
| Department |
Biology
|
| Lab |
Michael Rosbash
|
| Street address |
415 South Street
|
| City |
Waltham |
| State/province |
MA |
| ZIP/Postal code |
02454 |
| Country |
USA |
| |
|
| Platform ID |
GPL1322 |
| Series (2) |
| GSE40894 |
The Oscillating miRNA 959-964 cluster impacts Drosophila feeding time and other circadian outputs [expression]. |
| GSE40981 |
The Oscillating miRNA 959-964 cluster impacts Drosophila feeding time and other circadian outputs. |
|
