|
| Status |
Public on Mar 04, 2014 |
| Title |
Heart sample 100 |
| Sample type |
RNA |
| |
|
| Source name |
Mouse Heart
|
| Organism |
Mus musculus |
| Characteristics |
tissue: heart
Sex: M
cage: 4
maternal diet: LF
diet: LF
weight (g) 3 weeks: 10.46
weight (g) weaning: 24.9
glucose (mg/dl): 171
length (mm): 100
bmi: 0.249
genetic background: C57BL\6
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Data processing |
The data were normalised using quantile normalisation in Beadstudio
|
| |
|
| Submission date |
Sep 14, 2012 |
| Last update date |
Mar 04, 2014 |
| Contact name |
James Hester |
| Organization name |
Cleveland Clinic
|
| Department |
Genomic Medicine
|
| Lab |
Serre Lab
|
| Street address |
9500 Euclid Avenue
|
| City |
Cleveland |
| State/province |
Ohio |
| ZIP/Postal code |
44195 |
| Country |
USA |
| |
|
| Platform ID |
GPL6885 |
| Series (2) |
| GSE40898 |
Genome-wide analysis of heart gene expression induced by maternal diet |
| GSE40903 |
Genome-wide analysis of expression in various tissues in response to maternal diet |
|
