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Sample GSM1012270 Query DataSets for GSM1012270
Status Public on Feb 06, 2013
Title Control morpholino, uninjected side, replicate 1
Sample type RNA
 
Source name control morpholino, uninjected side
Organism Xenopus laevis
Characteristics tissue: embryo
treatment: control morpholino
side: uninjected
Treatment protocol Morpholinos were injected in two neighbouring, animal or vegetal blastomeres on one side of the embryo, at half the dose (i.e. 40 ng total).
Growth protocol Xenopus laevis eggs were collected, fertilized in vitro and handled following standard procedures. Embryos were staged according to Nieuwkoop and Faber (1967).
Extracted molecule total RNA
Extraction protocol Total cellular RNA was isolated with TRIzol (Qiagen) and phenol/chloroform extraction. On-column RNA clean-up, including a DNase digestion step, was performed using the RNeasy-Mini-Kit (Qiagen).
Label biotin
Label protocol Standard Affymetrix protocol.
 
Hybridization protocol Standard Affymetrix protocol.
Scan protocol Standard Affymetrix protocol.
Description X. laevis embryos, control knockdown, uninjected side, replicate 1.
Data processing R/Bioconductor, gcrma normalization, default parameters.
 
Submission date Oct 01, 2012
Last update date Feb 06, 2013
Contact name Tobias Straub
E-mail(s) tstraub@med.uni-muenchen.de
Organization name LMU Munich
Department Biomedical Center, Bioinformatics
Street address GroƟhadener Str. 9
City Martinsried
ZIP/Postal code 82152
Country Germany
 
Platform ID GPL10756
Series (1)
GSE41256 Suv4-20h Histone Methyltransferases Promote Neuroectodermal Differentiation by Silencing the Pluripotency-Associated Oct-25 Gene

Data table header descriptions
ID_REF
VALUE GC-RMA expression value

Data table
ID_REF VALUE
AFFX-BioB-3_at 8.518701808
AFFX-BioB-5_at 8.39202674
AFFX-BioB-M_at 8.918459883
AFFX-BioC-3_at 10.40651988
AFFX-BioC-5_at 9.865035037
AFFX-BioDn-3_at 12.23489194
AFFX-BioDn-5_at 11.80828454
AFFX-CreX-3_at 14.23608785
AFFX-CreX-5_at 14.05805244
AFFX-DapX-3_at 11.48620122
AFFX-DapX-5_at 8.285214409
AFFX-DapX-M_at 10.27454004
AFFX-LysX-3_at 8.311644607
AFFX-LysX-5_at 6.316517193
AFFX-LysX-M_at 6.811235634
AFFX-PheX-3_at 8.260602389
AFFX-PheX-5_at 6.817214498
AFFX-PheX-M_at 7.648661981
AFFX-r2-Bs-dap-3_at 11.64313841
AFFX-r2-Bs-dap-5_at 8.749819113

Total number of rows: 32635

Table truncated, full table size 947 Kbytes.




Supplementary file Size Download File type/resource
GSM1012270_C_MO_80_UNINJ_Exp1.CEL.gz 3.2 Mb (ftp)(http) CEL
Processed data included within Sample table

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