|
| Status |
Public on Feb 06, 2013 |
| Title |
Control morpholino, uninjected side, replicate 1 |
| Sample type |
RNA |
| |
|
| Source name |
control morpholino, uninjected side
|
| Organism |
Xenopus laevis |
| Characteristics |
tissue: embryo
treatment: control morpholino
side: uninjected
|
| Treatment protocol |
Morpholinos were injected in two neighbouring, animal or vegetal blastomeres on one side of the embryo, at half the dose (i.e. 40 ng total).
|
| Growth protocol |
Xenopus laevis eggs were collected, fertilized in vitro and handled following standard procedures. Embryos were staged according to Nieuwkoop and Faber (1967).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total cellular RNA was isolated with TRIzol (Qiagen) and phenol/chloroform extraction. On-column RNA clean-up, including a DNase digestion step, was performed using the RNeasy-Mini-Kit (Qiagen).
|
| Label |
biotin
|
| Label protocol |
Standard Affymetrix protocol.
|
| |
|
| Hybridization protocol |
Standard Affymetrix protocol.
|
| Scan protocol |
Standard Affymetrix protocol.
|
| Description |
X. laevis embryos, control knockdown, uninjected side, replicate 1.
|
| Data processing |
R/Bioconductor, gcrma normalization, default parameters.
|
| |
|
| Submission date |
Oct 01, 2012 |
| Last update date |
Feb 06, 2013 |
| Contact name |
Tobias Straub |
| E-mail(s) |
tstraub@med.uni-muenchen.de
|
| Organization name |
LMU Munich
|
| Department |
Biomedical Center, Bioinformatics
|
| Street address |
Großhadener Str. 9
|
| City |
Martinsried |
| ZIP/Postal code |
82152 |
| Country |
Germany |
| |
|
| Platform ID |
GPL10756 |
| Series (1) |
| GSE41256 |
Suv4-20h Histone Methyltransferases Promote Neuroectodermal Differentiation by Silencing the Pluripotency-Associated Oct-25 Gene |
|
