|
| Status |
Public on Jul 26, 2021 |
| Title |
37 oC stress IR64 7-day-old seedling, Biological replicate 2 |
| Sample type |
RNA |
| |
|
| Source name |
7-day-old seedling
|
| Organism |
Oryza sativa Indica Group |
| Characteristics |
cultivar: IR64
stress: 37 oC heat stress
time: 1h
tissue: seedling
|
| Treatment protocol |
The seven-day-old seedings of each variety were subjected to heat stress by first pre-treating them at 37 oC for 1 h followed by 42 oC for 2 h at approximately 40 % relative humidity, respectively. Control tissues were maintained on RGM for 3 h at 28±1oC.
|
| Growth protocol |
Seeds of each variety were surface sterilized with 70% ethanol followed by 0.1 % HgCl2 with repeated rinses with Mili-Q water, and were allowed to imbibe in water for 16 h. The seedlings were grown hydroponically for seven days on rice growth medium (RGM), pH 4.8.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA of whole seedlings was performed according to the manufacturer's instructions.
|
| Label |
Biotin
|
| Label protocol |
Biotinylated aRNA were prepared according to the revised 3' IVT Affymetrix protocol from 500ng total RNA.
|
| |
|
| Hybridization protocol |
Following fragmentation, 12.5 ug of aRNA were hybridized for 16 h at 450C on GeneChip Rice Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix Gene Chip Scanner 3300.
|
| Description |
Gene expression data of 37 oC for 1 h heat stressed 7-day-old IR64 seedling
|
| Data processing |
First-order analysis was performed using Affymetrix Gene Chip Operating (GCOS) software 1.2.1. Normalization of data and differential expression analysis was done using ArrayAssist ver 5.5 (Stratagene, CA, USA).
|
| |
|
| Submission date |
Oct 17, 2012 |
| Last update date |
Jul 26, 2021 |
| Contact name |
Jitendra Paul Khurana |
| E-mail(s) |
khuranaj@genomeindia.org
|
| Phone |
91 11 24115126
|
| Organization name |
University of Delhi South Campus
|
| Department |
Department of Plant Molecular Biology
|
| Street address |
Benito Juarez Road
|
| City |
New Delhi |
| ZIP/Postal code |
110021 |
| Country |
India |
| |
|
| Platform ID |
GPL2025 |
| Series (1) |
| GSE41648 |
Expression data for heat tolerant and susceptible cultivars of indica rice |
|
