|
| Status |
Public on May 01, 2013 |
| Title |
Control - 24h - rep1 |
| Sample type |
RNA |
| |
|
| Source name |
WB-F344 cell line
|
| Organism |
Rattus norvegicus |
| Characteristics |
cell line: stem-like WB-F344 cells
treatment: DMSO, 0.1% v/v
time of exposure: 24 hours
|
| Treatment protocol |
18 Total samples were analyzed (3 independent repeats for each treatment and time point): Control or PCB 126 for 6 h, 24 h, 72 h.
|
| Growth protocol |
Confluent WB-F344 cells (passages 14-16) were grown on 60 mm diameter dishes and treated with 100 nM PCB 126 or 0.1 % DMSO (control).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Cells were lysed and total RNA was extracted by Quiagen RNeasy Plus Mini Kit according to protocol provided.
|
| Label |
biotin
|
| Label protocol |
Samples were enzymatically fragmented and biotinylated using the WT Terminal Labeling Kit (Affymetrix)
|
| |
|
| Hybridization protocol |
Samples were hybridized using Affymetrix hybridization kit materials according to manufacturer's protocol.
|
| Scan protocol |
Affymetrix Gene Chip Scanner
|
| Description |
07_1_DMSO_24
|
| Data processing |
Affymetrix Power Tools 1.12.0 as implemented via FlexArray 1.6.1.1. Data were normalized using RMA algorithm.
Probe Group File: RaEx-1_0-st-v1.r2.pgf
Cel Layout File: RaEx-1_0-st-v1.r2.clf
|
| |
|
| Submission date |
Oct 29, 2012 |
| Last update date |
May 01, 2013 |
| Contact name |
Jan Vondracek |
| E-mail(s) |
vondracek@ibp.cz
|
| Organization name |
Institute of Biophysics ASCR
|
| Department |
Department of Cytokinetics
|
| Street address |
Kralovopolska 135
|
| City |
Brno |
| ZIP/Postal code |
61265 |
| Country |
Czech Republic |
| |
|
| Platform ID |
GPL6194 |
| Series (1) |
| GSE41902 |
Expression data from rat liver stem-like WB-F344 cells treated with 3,3’,4,4’,5-pentachlorobiphenyl (PCB 126) |
|
