|
| Status |
Public on Nov 16, 2012 |
| Title |
Central memory, biological replicate A |
| Sample type |
RNA |
| |
|
| Source name |
human purified peripheral blood CD8 central memory lymphocytes, untreated
|
| Organism |
Homo sapiens |
| Characteristics |
cell: unmanipulated CD8 central memory control cells
protocol: untreated
|
| Treatment protocol |
Cells were kept in culture in IMDM supplemented with 10% FBS and IL-7 and IL-15 (5 ng/ml)
|
| Growth protocol |
Purified CD8 T-cell subsets were either directly used for RNA extraction or activated anti-CD3/CD28-conjugated magnetic beads in 3:1 bead/T-cell ratio and then cultured with IL-7 and IL-15 (5 ng/ml) for 15 days.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared using the BioArrayTM HighYieldTM RNA Transcript Labeling Kit (ENZO Biochem, New York, NY) according to manufacturer's intructions.
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome HG-U133 Plus 2.0 arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
Standard Affymetrix scanning procedures on a GCS3000 7G Affymetrix scanner operated by GCOS, GeneChip Operating Software
|
| Description |
Gene expression data from control central memory CD8 cells
|
| Data processing |
RMA of .CEL files using affy Bioconductor library. In RMA, PM values have been background adjusted, normalized using quantile normalization, and log2 expression measure calculated using median polish summarization. Probes were summarized using the revised entrez-based probe annotation of Dai (Dai 2005, the package hgu133plus2hsentrezgcdf.db, version 13)
|
| |
|
| Submission date |
Oct 29, 2012 |
| Last update date |
Sep 01, 2016 |
| Contact name |
Silvio Bicciato |
| E-mail(s) |
silvio.bicciato@unipd.it
|
| Phone |
+39-049-827-6108
|
| Organization name |
University of Padova
|
| Department |
Molecular Medicine
|
| Street address |
via U. Bassi 59/b
|
| City |
Padova |
| ZIP/Postal code |
35131 |
| Country |
Italy |
| |
|
| Platform ID |
GPL14877 |
| Series (1) |
| GSE41909 |
IL-7 and IL-15 instruct the generation of human memory stem T cells from naïve precursors |
|
| Relations |
| Reanalyzed by |
GSE86362 |
