|
| Status |
Public on Nov 01, 2012 |
| Title |
Mouse 6 |
| Sample type |
RNA |
| |
|
| Source name |
Brown adipose tissue
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57Bl6N
Sex: Male
temperature: 21ºC
age: 8 weeks
|
| Treatment protocol |
Groups of mice were either kept at room temperature (RT) or cold exposure (8C for 24 hours)
|
| Growth protocol |
Primary tissue was taken from 8 week old mive
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using the Trizol method
|
| Label |
Cy3
|
| Label protocol |
Cy3 labeling of RNA was performed according to recommendations provided by Agilent (see CXR Biosciences website)
|
| |
|
| Hybridization protocol |
100 ng reference RNA was dephosphorylated by incubation with calf intestinal phosphatase at 37°C for 30 min and denatured using 100% DMSO at 100°C for 5 min. Samples were labelled with pCp-Cy3 using T4 ligase by incubation at 16°C for 1 hour. Each labelled RNA sample was hybridised to an individual array on 8_x_15K format Agilent mouse miRNA array slides, with each array containing probes for 567 mouse miRNAs
|
| Scan protocol |
Arrays were scanned on an Agilent scanner according to recommendations provided by Agilent (see CXR Biosciences website)
|
| Description |
Total RNA was extracted from brown adipose tissue using the Trizol extraction method.
|
| Data processing |
Data were acquired using Agilent Feature Extraction software version 9.5.3.1. US22502575_251911911060_S01_miRNA_107_Sep09_1_3.txt array yielded a very poor hybridization signal and did not pass the Agilent quality control metrics in the Feature Extraction software. The signature lists were produced using the resolver error weighted mean.
|
| |
|
| Submission date |
Oct 31, 2012 |
| Last update date |
Nov 01, 2012 |
| Contact name |
Markus Stoffel |
| E-mail(s) |
stoffel@biol.ethz.ch
|
| Organization name |
ETH Zurich
|
| Department |
Molecular Health Science
|
| Lab |
Metabolic Diseases
|
| Street address |
Schafmattstr. 22
|
| City |
Zurich |
| State/province |
ZH |
| ZIP/Postal code |
8093 |
| Country |
Switzerland |
| |
|
| Platform ID |
GPL7732 |
| Series (1) |
| GSE41306 |
Expression Profiles of miRNAs in brown fat of mice at room temperature and cold exposure (8C) |
|
