|
| Status |
Public on May 01, 2013 |
| Title |
Tissue from normal bladder_Normal_2 |
| Sample type |
RNA |
| |
|
| Source name |
Tissue from normal bladder
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: normal bladder
|
| Treatment protocol |
NA
|
| Growth protocol |
NA
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to manufacturer's protocol
|
| Label |
biotin
|
| Label protocol |
10 ug of Qiagen-purified total RNA was labeled with 6ul of 1mg/ml R-Phycoerythrin Streptavidin (SAPE)
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133 Plus 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 7G
|
| Description |
Gene expression data from Tissue from normal bladder
|
| Data processing |
The data were analyzed with custom CDF environments created by the MBNI - The version 10 entrez gene ID CDF was used. Data were then rma normalized using R and the bioconductor suite of analysis tools (eset <- rma(ab))
|
| |
|
| Submission date |
Nov 06, 2012 |
| Last update date |
May 01, 2013 |
| Contact name |
Jacob Zhang |
| E-mail(s) |
zjz999@gmail.com
|
| Phone |
6166358181
|
| Organization name |
Van Andel Institute
|
| Department |
Cancer Genetics
|
| Street address |
333 Bostwick Ave
|
| City |
Grand Rapids |
| State/province |
MI |
| ZIP/Postal code |
49503 |
| Country |
USA |
| |
|
| Platform ID |
GPL9828 |
| Series (1) |
| GSE42089 |
The investigational Aurora kinase A inhibitor MLN8237 induces defects in cell viability and cell cycle progression in mouse bladder cancer cells in vitro and in vivo |
|
