|
| Status |
Public on Nov 07, 2012 |
| Title |
HESC_Diff_S5_87.926.3.1 [mRNA] |
| Sample type |
RNA |
| |
|
| Source name |
embryonic stem cells, differentiated; day 18
|
| Organism |
Homo sapiens |
| Characteristics |
loring pipeline sample id: 87.926.3.1
loring pipeline stem cell matrix version: 557
cell line: WA09
cell type: embryonic stem cells
passage number: 50
differentiation stage: differentiated; day 18
gender: female
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was purified using the MirVana RNA extraction kit (Ambion), quantified using the Ribogreen reagent (Lifetech, Inc.), and quality-controlled on a Bioanalyzer (Agilent).
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays.
|
| |
|
| Hybridization protocol |
The labeled product was hybridized to Illumina HT-12 v4 arrays.
|
| Scan protocol |
Scanned on a BeadArray Reader (Illumina, Inc.) according to the manufacturer's instructions.
|
| Description |
HESC _Diff_S5_87.926.3.1
|
| Data processing |
The data were filtered for detection pValue > 0.01 and normalised using robust spline normalisation with lumi in R.
|
| |
|
| Submission date |
Nov 06, 2012 |
| Last update date |
Aug 09, 2013 |
| Contact name |
Xiaoyan Liao |
| E-mail(s) |
xliao@scripps.edu
|
| Phone |
858-784-7753
|
| Organization name |
University of California, San Diego
|
| Department |
Reproductive Medicine
|
| Lab |
Louise Laurent
|
| Street address |
200 W Arbor Street
|
| City |
La Jolla |
| State/province |
CA |
| ZIP/Postal code |
92037 |
| Country |
USA |
| |
|
| Platform ID |
GPL10558 |
| Series (2) |
| GSE42094 |
Dynamic regulation of miRNA and mRNA signatures during in vitro pancreatic differentiation (mRNA) |
| GSE42095 |
Dynamic regulation of miRNA and mRNA signatures during in vitro pancreatic differentiation |
|
