|
| Status |
Public on Sep 01, 2013 |
| Title |
Pt8_IL21 |
| Sample type |
RNA |
| |
|
| Source name |
Pt8_IL21
|
| Organism |
Homo sapiens |
| Characteristics |
patient: 8
disease state: CLL
cell type: CD40-activated CLL cells
agent: IL21
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
4686257010_L
|
| Data processing |
The data were processed and normalised using quantile normalisation with BeadStudio software
|
| |
|
| Submission date |
Nov 08, 2012 |
| Last update date |
Sep 01, 2013 |
| Contact name |
Loris De Cecco |
| E-mail(s) |
loris.dececco@istitutotumori.mi.it
|
| Organization name |
IRCSS Istituto Nazionale Tumori
|
| Street address |
via Venezian 1
|
| City |
Milan |
| ZIP/Postal code |
20133 |
| Country |
Italy |
| |
|
| Platform ID |
GPL8179 |
| Series (2) |
| GSE42160 |
IL-21 regulates miRNA expression in B-chronic lymphocytic leukemia |
| GSE42193 |
IL-21 controls gene expression in B-chronic lymphocytic leukemia (CLL) cells through micro-RNA regulation |
|
