|
| Status |
Public on Jun 25, 2013 |
| Title |
Before treatment (week 0) IBDB27 |
| Sample type |
RNA |
| |
|
| Source name |
PBMC
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: PBMC
subject: Patient 5
response: NR - non-responder
treatment: Before treatment
time: week 0
disease state: Crohn's disease
|
| Treatment protocol |
Treatment was infliximab infusion at week 0 and at week 2.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Peripheral blood momonuclear cells were separated by Ficoll gradient centrifugation.
|
| Label |
biotin
|
| Label protocol |
Ambion WT Expression Kit (Applied Biosystems) and GeneChip WT Terminal Labeling and Control Kit (Affymetrix) were used for amplifying and labeling 250 ng of RNA samples.
|
| |
|
| Hybridization protocol |
Samples were hybridized at 45 degrees Celsius for 16 hours and then standard washing protocol was performed using GeneChip Fluidics Station 450.
|
| Scan protocol |
Arrays were scanned on GeneChip Scanner 7G (Affymetrix).
|
| Data processing |
Gene Spring GX 10 was used for data analysis, CEL files were imported using RMA algorithm and median normalization was performed. CHP files were generated using Affymetrix Expression Control.
|
| |
|
| Submission date |
Nov 14, 2012 |
| Last update date |
Jun 25, 2013 |
| Contact name |
Bertalan Mesko |
| E-mail(s) |
berci.mesko@gmail.com
|
| Organization name |
University of Debrecen
|
| Department |
Department of Biochemistry and Molecular Biology
|
| Lab |
Nuclear Hormone Receptor Research Laboratory
|
| Street address |
Egyetem tér 1.
|
| City |
Debrecen |
| ZIP/Postal code |
4012 |
| Country |
Hungary |
| |
|
| Platform ID |
GPL6244 |
| Series (1) |
| GSE42296 |
Distinct, non-overlapping gene panels of peripheral blood gene expression predict response to infliximab therapy in rheumatoid arthritis and Crohn's disease |
|
