|
| Status |
Public on Mar 31, 2014 |
| Title |
ResuscitatedAcutePancreatitis001 |
| Sample type |
RNA |
| |
|
| Source name |
Mesenteric lymph, taurocholate induced pancreatitis animals which also received fluid resuscitation
|
| Organism |
Rattus norvegicus |
| Characteristics |
strain: Wistar
weight: 360-500g
age: 70-110 days old
Sex: male
rna: enriched small RNA
tissue: mestenteric lymph
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted from 250ul mesenteric lymph using trizol and a Purelink microRNA kit (Life Technologies) and eluted in a volume of 30ul water
|
| Label |
biotin
|
| Label protocol |
8ul RNA was direct labelled using the Flashtag HSR kit as standard
|
| |
|
| Hybridization protocol |
biotinylated RNA was hybridised to GeneChip miRNA V1.0 arrays at 480C, 60rpm for 16 hours. GeneChips were washed and stained in the Affy Fluidics station 450.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
miRNA expression data from resuscitated acute pancreatitis lymph
|
| Data processing |
Affymetrix .CEL files were analysed using the statistical programming language R using the Bioconductor packages affy, affyPLM and limma. The data was preprocessed using RMA.
|
| |
|
| Submission date |
Nov 21, 2012 |
| Last update date |
Mar 31, 2014 |
| Contact name |
Cherie Blenkiron |
| E-mail(s) |
c.blenkiron@auckland.ac.nz
|
| Organization name |
University of Auckland
|
| Department |
Surgery
|
| Lab |
ASML
|
| Street address |
Private bag 92019
|
| City |
Auckland |
| ZIP/Postal code |
1142 |
| Country |
New Zealand |
| |
|
| Platform ID |
GPL8786 |
| Series (1) |
| GSE42455 |
microRNA expression in Mestenteric lymph samples from rat models of acute pancreatitis |
|
