|
| Status |
Public on Aug 01, 2013 |
| Title |
F10_HT_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
LPI-5_F10_35°C
|
| Organism |
Populus tremula x Populus alba |
| Characteristics |
genotype/variation: transgenic Populus expressing plastidic Irp9
transgenic line: FD-Irp9 line F10
temperature: high (35°C)
tissue: young source leaves at leaf plastochron index (LPI) 5
|
| Treatment protocol |
Plants in one chamber were exposed to HT (35°C/25°C, day/night)) for one week, while those in the other chamber remained at NT (27°C/17°C, day/night). Leaf samples were harvested for RNA extraction.
|
| Growth protocol |
Vegetatively propagated plants were randomly distributed to two identical chambers and grown at NT (27°C/17°C, day/night) to a height of ~120 cm.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
CTAB protocol
|
| Label |
Cy3
|
| Label protocol |
Agilent standard protocol
|
| |
|
| Hybridization protocol |
Agilent standard protocol
|
| Scan protocol |
Slides were scanned on an Agilent DNA Microarray Scanner at 3 µm resolution
|
| Description |
expression data from transgenic line F10 at HT
F10HT2
|
| Data processing |
The scanned images were analyzed with Feature Extraction Software 9.1 (Agilent) using default parameters to obtain background subtracted Processed Signal intensities(gProcessedSignal). Percentile Shift (75th) method was applied for normalization with Agilent's GeneSpring GX software
|
| |
|
| Submission date |
Nov 26, 2012 |
| Last update date |
Aug 01, 2013 |
| Contact name |
Chung-Jui Tsai |
| E-mail(s) |
cjtsai@uga.edu
|
| Organization name |
University of Georgia
|
| Department |
School of Forestry and Natural Resources
|
| Street address |
120 Green Street
|
| City |
Athens |
| State/province |
GA |
| ZIP/Postal code |
30606 |
| Country |
USA |
| |
|
| Platform ID |
GPL16322 |
| Series (1) |
| GSE42511 |
Transcriptional profiling of Populus leaves with altered SA levels |
|
