|
| Status |
Public on Nov 27, 2012 |
| Title |
PR9692 expressing control shRNA, biological rep2 |
| Sample type |
RNA |
| |
|
| Source name |
PR9692 cells stably transfected with control shRNA
|
| Organism |
Gallus gallus |
| Characteristics |
cell line: PR9692
tranfection: control shRNA
strain: CC.R1
|
| Growth protocol |
All the PR9692 derived cell lines were grown in Iscove´s modified Dulbecco´s minimal essential medium supplemented with 8% fetal calf serum, 2% chicken serum, and antibiotics. The cells were cultured at 40°C in a humidified atmosphere with 5% CO2.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Approximately 10 millions cultured cells were dissolved in 2 ml of RNAzol (Sigma-aldrich) and total RNA was extracted according to manufacturer's protocol.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Chicken Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
|
| Description |
Gene expression data from sarcoma derived PR9692 cell line stably transfected with control shRNA
|
| Data processing |
The data were analyzed with Partek Genomics Suite (version 6.4) using Affymetrix default annotation and GC-RMA as normalization method.
|
| |
|
| Submission date |
Nov 26, 2012 |
| Last update date |
Nov 27, 2012 |
| Contact name |
Vladimir Cermak |
| E-mail(s) |
cermak@img.cas.cz
|
| Organization name |
Institute of Molecular Genetics, Czech Academy of Sciences
|
| Lab |
Molecular Virology
|
| Street address |
Videnska 1083
|
| City |
Prague |
| ZIP/Postal code |
14220 |
| Country |
Czech Republic |
| |
|
| Platform ID |
GPL3213 |
| Series (1) |
| GSE42516 |
Downregulation of the HOPX gene decreases metastatic activity in a chicken sarcoma cell line model and identifies genes associated with metastasis |
|
