|
| Status |
Public on Apr 24, 2013 |
| Title |
Gfi1 |
| Sample type |
SRA |
| |
|
| Source name |
MAST
|
| Organism |
Mus musculus |
| Characteristics |
cell type: Mast cell
chip antibody: Gfi1
chip antibody manufacturer: Abcam
chip antibody catalog #: ab21061
genetic background: B6CBA/F1 (a cross between a C57BL/6 and a CBA)
|
| Growth protocol |
Bone marrow cells were collected from tibias and femurs of 3- to 5- month old adult mice. Cells were cultured in Iscove’s modified Dulbecco’s medium (IMDM) supplemented with 10% fetal bovine serum (Sigma), 1% penicillin/streptomycin (Sigma), 150 micromolar MTG (Sigma), 10% stem cell factor conditional media from BHK/MKL cells and 10ng/ml of recombinant mIL-3 (Peprotech). Cells were frequently transferred to new flasks to remove adherent cells and experiments were performed after 3 weeks, when cultures were homogenous. Homogeneity of culture was confirmed by presence of FcERI by FACS and toluidine blue staining of cytospins.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
ChIP assays were performed as previously described (Wilson NK et al., 2010 Cell Stem Cell) using polyclonal antibodies against GATA2 (Santa Cruz, sc9008x) and GFI1 (Abcam, ab21061).
Each sample was amplified and sequenced Illumina's instructions.
|
| |
|
| Library strategy |
ChIP-Seq |
| Library source |
genomic |
| Library selection |
ChIP |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Description |
Chromatin Immunoprecipitated genomic DNA
wildtype
|
| Data processing |
Reads aligned to mm9 using Bowtie
bedGraphs created in-house
Genome_build: mm9
Supplementary_files_format_and_content: bedGraph – read density profiles
|
| |
|
| Submission date |
Nov 26, 2012 |
| Last update date |
May 15, 2019 |
| Contact name |
Rebecca Louise Hannah |
| E-mail(s) |
rlh60@cam.ac.uk, rebeccahannah@live.co.uk
|
| Organization name |
University of Cambridge
|
| Department |
Haematology
|
| Street address |
Addenbrookes Hospital, Hills Road
|
| City |
Cambridge |
| ZIP/Postal code |
CB2 0XY |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL13112 |
| Series (1) |
| GSE42518 |
High-throughput single cell gene expression analysis in primary blood stem and progenitor cells identifies new links between key transcriptional regulators |
|
| Relations |
| SRA |
SRX206425 |
| BioSample |
SAMN01818988 |
