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Status
Public on Apr 18, 2006
Title
WDG14-29 recA untr Rep1
Sample type
RNA
Channel 1 Source name
recA untreated
Organism
Bacillus subtilis Characteristics
RNA from untreated recA cells. repetition1
Treatment protocol
Au et al.
Growth protocol
Au et al.
Extracted molecule
total RNA
Extraction protocol
Goranov AI, Katz L, Breier AM, Burge CB, Grossman AD. A transcriptional response to replication status mediated by the conserved bacterial replication protein DnaA.
Label
Cy5
Label protocol
Goranov AI, Katz L, Breier AM, Burge CB, Grossman AD. A transcriptional response to replication status mediated by the conserved bacterial replication protein DnaA.
Channel 2 Source name
pooled reference RNA
Organism
Bacillus subtilis Characteristics
pooled RNA form cells grown under various conditions
Extracted molecule
total RNA
Label
Cy3
Description
Comparison of RNA from untreated recA cells with reference RNA
Data processing
Ratio of Cy5/Cy3 normalized to total signal. Only spots with >80% pixesl above background were analyzed
Submission date
Apr 10, 2006
Last update date
Apr 18, 2006
Contact name
Alan D. Grossman
E-mail(s)
clee2@mit.edu, adg@mit.edu Phone
617-253-6702
Organization name
MIT
Department
Biology
Lab
Grossman
Street address
31 Ames Street, 68-530
City
Cambridge
State/province
MA
ZIP/Postal code
02142
Country
USA
Platform ID
GPL3645 Series (1)
GSE4673 Analysis of the transcriptional response to DNA damage and replication arrest
Data table header descriptions ID_REF VALUE Ratio Cy5/Cy3 normalized to total signal (no background subtraction)
signal_Cy5 signal at 635nm
Background_Cy5_Mean background at 635nm
signal_Cy3 signal at 532nm
Background_Cy3_Mean background at 532nm
Data table ID_REF VALUE signal_Cy5 Background_Cy5_Mean signal_Cy3 Background_Cy3_Mean WDG14-3732
-0.013532434
82320
63
112080
67
WDG14-3825
-0.025957787
102804
66
141180
65
WDG14-3239
0.457406353
81480
67
80040
67
WDG14-3862
-0.338836614
171360
68
292320
70
WDG14-4558
-0.02628537
316784
76
435136
83
WDG14-3929
0.357680958
207480
63
218400
63
WDG14-2217
0.140477391
685680
68
839040
71
WDG14-3970
0.64225995
562848
76
486408
83
WDG14-2962
0.243950358
843024
59
960180
64
WDG14-1610
0.37783465
362880
64
376680
62
WDG14-2324
-0.273120731
106680
63
173880
64
WDG14-1178
-0.927670976
529680
58
1359000
68
WDG14-188
-0.267316204
27720
67
45000
66
WDG14-472
0.183754026
72384
70
85956
67
WDG14-1048
-0.198094808
37908
67
58656
70
WDG14-44
-0.184133241
45708
68
70044
69
WDG14-328
0.216175504
64800
67
75240
68
WDG14-4120
-0.949573913
340392
53
886704
59
WDG14-224
-0.09612389
44280
69
63840
68
WDG14-3152
1.05620068
510240
65
330960
66
4122 170 Kbytes .Supplementary data files not provided
