|
| Status |
Public on Mar 07, 2013 |
| Title |
donor 2 control conditions |
| Sample type |
RNA |
| |
|
| Source name |
CD4+ T cells differentiated under control conditions, day7
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: lymphocyte
|
| Treatment protocol |
plate bound aCD3 (10µg/ml) and soluble aCD28 (1µg/ml) and soluble IL-1beta (12.5ng/ml), IL-6 (25ng/ml), IL-21 (25ng/ml), IL-23 (25ng/ml) and TGF-beta1(5ng/ml) in the presence (nacl) or absence (none) of additional 40mM NaCl in X-VIVO cell culture media (LONZA). RNA was extracted after 7 days of culture.
|
| Growth protocol |
FACS sorted naive human CD4+ T cells (CD45RA+CD45RO-CD25-CD127+) were differentiated into Th17 cells by stimulation with aCD3 and aCD28 and IL-1beta, IL-6, IL-21, IL-23 and TGF-beta1 in the presence (nacl) or absence (none) of additional 40mM NaCl. RNA was extracted after 7 days of culture.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted by Trizol according to the manufacturer (Invitrogen).
|
| Label |
biotin
|
| Label protocol |
Standard 3´IVT and biotin labelling at Yale Center for Genome Analysis (YCGA) according to Affymetrix guidelines.
|
| |
|
| Hybridization protocol |
Standard hybridisation at Yale Center for Genome Analysis (YCGA) for 16 hours at 45C to Affymetrix HG-U133 plus 2.0 arrays according to Affymetrix guidelines.
|
| Scan protocol |
Standard scan at Yale Center for Genome Analysis (YCGA) according to Affymetrix guidelines.
|
| Description |
Gene expression data from naive CD4+ T cells differentiated into Th17 cells
|
| Data processing |
The image data were analyzed using Affymetrix Command Console Viewer and metric analyses was carried out according to the instructions provided by Affymetrix using the Affymetrix Expression Console and standard MAS5.0 processing at Yale Center for Genome Analysis (YCGA).
|
| |
|
| Submission date |
Nov 28, 2012 |
| Last update date |
Sep 01, 2016 |
| Contact name |
Markus Kleinewietfeld |
| Organization name |
Yale
|
| Street address |
300 George St
|
| City |
New Haven |
| ZIP/Postal code |
06511 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE42569 |
Gene expression analysis of human CD4+ T cells differentiated into Th17 cells in the presence of high-salt |
|
| Relations |
| Reanalyzed by |
GSE86362 |
