|
| Status |
Public on Sep 27, 2013 |
| Title |
siAgo1 |
| Sample type |
RNA |
| |
|
| Source name |
PC-3 cells
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: PC-3
treatment: siAgo1
|
| Treatment protocol |
PC-3 cells were mock transfected or transfected with a pool of control siRNAs (siControl) or Ago1 siRNAs (siAgo1) at 10 nM total concentration for 48 hrs
|
| Growth protocol |
PC-3 cells cultured at exponential phase
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total cellular RNA was isolated using RNeasy kit. The resulting total RNA concentration and quality were assessed by the Agilent™ Bioanalyzer
|
| Label |
biotin
|
| Label protocol |
Ovation Pico WTA V2 & Encore Biotin Module (NuGen) were used to prepare probes for hybridization to Affymetrix GeneChip Human Gene 1.0 ST Arrays
|
| |
|
| Hybridization protocol |
Hybridization, washes, and scanning were performed by following the GeneChip Expression Analysis Technical Manual 702232 Rev. 3
|
| Scan protocol |
GeneChip scanner GCS3000
|
| Description |
Ago1 siRNA transfection
|
| Data processing |
Affymetrix GeneChip Command Console. Version 3.2.2. RMA
|
| |
|
| Submission date |
Nov 28, 2012 |
| Last update date |
Sep 27, 2013 |
| Contact name |
Vera Huang |
| E-mail(s) |
vhuang2013@gmail.com
|
| Organization name |
UCSF
|
| Department |
Urology
|
| Lab |
Long-Cheng Li Lab
|
| Street address |
1450 Third Street, Helen Diller Cancer Research Building, Room 302
|
| City |
San Francisco |
| State/province |
CA |
| ZIP/Postal code |
94158 |
| Country |
USA |
| |
|
| Platform ID |
GPL6244 |
| Series (1) |
| GSE42600 |
Gene expression profiling in cells with Ago1 knockdown (Affymetrix) |
|
