|
| Status |
Public on Nov 28, 2015 |
| Title |
hiPSC line FiPS6-3, passage 14 ENSE |
| Sample type |
RNA |
| |
|
| Source name |
human induced pluripotent stem cell line FiPS6-3, passage 14
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: human induced pluripotent stem cell line
cell line/clone: clone FiPS6-3
passage: 14
cell origin: cell line is derived from Human neonatal foreskin fibroblast HFF cell, passage #11
|
| Treatment protocol |
none
|
| Growth protocol |
Cell lines were grown according to standard conditions.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated from cell pellets using Rneasy Kit (Qiagen). To eliminate genomic DNA from the samples Dnase I (Qiagen) digestion in column was included.
|
| Label |
biotin
|
| Label protocol |
accordingly to the manufacturer's instructions (Affymetrix)
|
| |
|
| Hybridization protocol |
accordingly to the manufacturer's instructions (Affymetrix)
|
| Scan protocol |
accordingly to the manufacturer's instructions (Affymetrix)
|
| Description |
Hybridized in Finnish Microarray and Sequencing Centre (Turku, Finland)
|
| Data processing |
Probe values were directly linked to Ensembl genes (build 49) using alternative CDF-files provided by brainarray (http://brainarray.mbni.med.umich.edu/), version 11. RMA-pre-processing was used to preprocess gene, transcript and exon values of Exon array values.
HuEx10stv2_Hs_ENSG, HuEx10stv2_Hs_ENST, HuEx10stv2_Hs_ENSE
HuEx10stv2_Hs_ENSG, HuEx10stv2_Hs_ENST, HuEx10stv2_Hs_ENSE
|
| |
|
| Submission date |
Nov 29, 2012 |
| Last update date |
Nov 28, 2015 |
| Contact name |
Reija Autio |
| Organization name |
Tampere University of Technology
|
| Department |
SGN
|
| Street address |
Korkeakoulunkatu 1
|
| City |
Tampere |
| ZIP/Postal code |
33101 |
| Country |
Finland |
| |
|
| Platform ID |
GPL16341 |
| Series (1) |
| GSE42625 |
Affymetrix Human Exon 1.0 ST Array data for human induced pluripotent stem cells (hiPSCs) and human fibroblasts |
|
| Relations |
| Alternative to |
GSM1046238 (gene) |
