|
| Status |
Public on Apr 18, 2006 |
| Title |
WDG14-17 phage- recA untreated repl2 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
phage- recA untreated
|
| Organism |
Bacillus subtilis |
| Characteristics |
YB3000 grown in minimal medium. replicate 2
|
| Treatment protocol |
Au et al. Genetic composition of the Bacillus subtilis SOS system.J Bacteriol. 2005 Nov;187(22):7655-66.
|
| Growth protocol |
Goranov et al. A transcriptional response to replication status mediated by the conserved bacterial replication protein DnaA.Proc Natl Acad Sci U S A. 2005 Sep 6;102(36):12932-7. Epub 2005 Aug 24.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Goranov et al. A transcriptional response to replication status mediated by the conserved bacterial replication protein DnaA.Proc Natl Acad Sci U S A. 2005 Sep 6;102(36):12932-7.
|
| Label |
Cy5
|
| Label protocol |
Goranov et al. A transcriptional response to replication status mediated by the conserved bacterial replication protein DnaA.Proc Natl Acad Sci U S A. 2005 Sep 6;102(36):12932-7.
|
| |
|
| Channel 2 |
| Source name |
reference RNA
|
| Organism |
Bacillus subtilis |
| Characteristics |
pooled RNA sample
|
| Treatment protocol |
Goranov et al. A transcriptional response to replication status mediated by the conserved bacterial replication protein DnaA.Proc Natl Acad Sci U S A. 2005 Sep 6;102(36):12932-7.
|
| Growth protocol |
Goranov et al. A transcriptional response to replication status mediated by the conserved bacterial replication protein DnaA.Proc Natl Acad Sci U S A. 2005 Sep 6;102(36):12932-7.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Goranov et al. A transcriptional response to replication status mediated by the conserved bacterial replication protein DnaA.Proc Natl Acad Sci U S A. 2005 Sep 6;102(36):12932-7.
|
| Label |
Cy3
|
| Label protocol |
Goranov et al. A transcriptional response to replication status mediated by the conserved bacterial replication protein DnaA.Proc Natl Acad Sci U S A. 2005 Sep 6;102(36):12932-7.
|
| |
|
| |
| Hybridization protocol |
Goranov et al. A transcriptional response to replication status mediated by the conserved bacterial replication protein DnaA.Proc Natl Acad Sci U S A. 2005 Sep 6;102(36):12932-7.
|
| Description |
hybridization of phage- recA RNA with reference RNA sample
|
| Data processing |
Ratio Cy5/Cy3 normalized to total signal (no background subtraction).Only spots with >80% pixels above background were analyzed
|
| |
|
| Submission date |
Apr 10, 2006 |
| Last update date |
Apr 18, 2006 |
| Contact name |
Alan D. Grossman |
| E-mail(s) |
clee2@mit.edu, adg@mit.edu
|
| Phone |
617-253-6702
|
| Organization name |
MIT
|
| Department |
Biology
|
| Lab |
Grossman
|
| Street address |
31 Ames Street, 68-530
|
| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02142 |
| Country |
USA |
| |
|
| Platform ID |
GPL3645 |
| Series (1) |
| GSE4673 |
Analysis of the transcriptional response to DNA damage and replication arrest |
|
