|
| Status |
Public on Dec 05, 2012 |
| Title |
WT incubated with 1mM copper sulfate, biological rep1 |
| Sample type |
RNA |
| |
|
| Source name |
C. elegans WT animal incubated with copper sulfate for 1 hour
|
| Organism |
Caenorhabditis elegans |
| Characteristics |
strain: N2 Bristol
genotype: wildtype
age: 1 day adult
|
| Treatment protocol |
Worms were collected with H2O and incubated with H2O or 1mM copper sulfate for 1 hour at room temperature. Then Worms were washed by H2O and crushed in the Trizol solution (invitrogen) with beads.
|
| Growth protocol |
WT and kgb-1 mutant were maintained on nematode growth medium (NGM) plates at 20°C and fed with bacteria of the OP50 strain. Adult worms of each strain were incubated with H2O or 1 mM copper sulfate for 1 hour.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 250 ng total RNA (GeneChip 3’ IVT Express Kit User Manual, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, cRNA were hybridized for 16 hr at 45C on GeneChip C.elegans Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
|
| Description |
Gene expression data from WT adult worm incubated with copper sulfate for 1 hr.
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
|
| |
|
| Submission date |
Dec 04, 2012 |
| Last update date |
Dec 05, 2012 |
| Contact name |
Ayuna Hattori |
| Organization name |
Nagoya University
|
| Department |
Department of Molecular Biology
|
| Lab |
Matsumoto Kunihiro
|
| Street address |
Chikusa
|
| City |
Nagoya |
| State/province |
Aichi |
| ZIP/Postal code |
488-0804 |
| Country |
Japan |
| |
|
| Platform ID |
GPL200 |
| Series (1) |
| GSE42703 |
Expression data from C. elegans in the presence or absence of copper sulfate |
|
