|
| Status |
Public on Dec 06, 2012 |
| Title |
Alveolar rhabdomyosarcoma cell line Rh10 |
| Sample type |
RNA |
| |
|
| Source name |
Alveolar rhabdomyosarcoma cell line Rh10
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: Rh10
cell type: Alveolar rhabdomyosarcoma
|
| Treatment protocol |
No treatment was applied
|
| Growth protocol |
Cell lines were grown under standard growth conditions in DME supplemented with 10% fetal bovine serum, 2mM L-glutamine and 100 U/ml penicillin/streptomycin for 2 days
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated using the RNeays Kit from Qiagen according to the instructions from the manufacturer
|
| Label |
Biotin
|
| Label protocol |
cDNA synthesis and in vitro-transcription was done with Ambion´s Message Amp II 96 Kit (Cat. No. 1819) according to the manufacturers’ instructions, starting with 1 μg of total RNA.
|
| |
|
| Hybridization protocol |
Hybridization solution was prepared according to Affymetrix´ GeneChip Expression Analysis Technical Manual. The final concentration of cRNA is 60 ng/μl.Hybridization was performed for 16-18 hours at 45°C and 60 rpm in a rotating hybridization oven (Hybridization Oven 640, Affymetrix).
|
| Scan protocol |
Scanning was performed with the GeneChip Scanner 3000 7G (Affymetrix), which is controlled by Affymetrix´ software GeneChip Operating System (GCOS) v1.4
|
| Description |
Gene expression data from alveolar rhabdomyosarcoma cell line Rh10
|
| Data processing |
CEL-Files were imported into d-Chip software and expression values and ABS calls were exported
|
| |
|
| Submission date |
Dec 05, 2012 |
| Last update date |
Dec 06, 2012 |
| Contact name |
Beat W Schafer |
| E-mail(s) |
beat.schaefer@kispi.uzh.ch
|
| Phone |
+41442667553
|
| Organization name |
University Childrens Hospital
|
| Street address |
Steinwiesstrasse 75
|
| City |
Zurich |
| ZIP/Postal code |
8032 |
| Country |
Switzerland |
| |
|
| Platform ID |
GPL571 |
| Series (1) |
| GSE42750 |
Expression data from alveolar rhabdomyosarcoma cell lines |
|
