|
| Status |
Public on Dec 09, 2013 |
| Title |
mutant-batch-1-rep-2 |
| Sample type |
RNA |
| |
|
| Source name |
mutant-batch-1-rep-2
|
| Organism |
Borrelia hermsii |
| Characteristics |
genotype/variation: DAH motility mutant
|
| Treatment protocol |
no treatments, just strain differences
|
| Growth protocol |
Samples were prepared from 4 biological replicates of 50 ml broth cultures (5 x 107 cells/ml) of Borrelia hermsii
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from 50 ml B. hermsii DAH cultures (5 x 107 cells/ml) using the RNeasy kit (Qiagen, Inc., Valencia, CA) and contaminating DNA was removed using the DNA-free kit (Ambion, Inc, Austin, TX).
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol
|
| |
|
| Hybridization protocol |
All cRNAs were hybridized to a custom Affymetrix GeneChip
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 7GPlus.
|
| Description |
Gene expression data from mutant-batch-1-rep-2
|
| Data processing |
The data were analyzed with Expression Console
|
| |
|
| Submission date |
Dec 12, 2012 |
| Last update date |
Dec 09, 2013 |
| Contact name |
Dan Sturdevant |
| E-mail(s) |
dsturdevant@niaid.nih.gov
|
| Phone |
4063639248
|
| Organization name |
NIH
|
| Department |
NIAID
|
| Lab |
RTS
|
| Street address |
903 S 4th street
|
| City |
Hamilton |
| State/province |
MT |
| ZIP/Postal code |
59840 |
| Country |
USA |
| |
|
| Platform ID |
GPL7246 |
| Series (1) |
| GSE42863 |
Flagellar export apparatus protein, FliH, is involved in post-transcriptional regulation of FlaB, motility and virulence of the relapsing fever spirochete Borrelia hermsii |
|
