NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM105318 Query DataSets for GSM105318
Status Public on Apr 14, 2006
Title MCF7/BUS, hormone starvation, day 0, biological replication 2
Sample type RNA
 
Source name MCF7/BUS cells, human breast adenocarcinoma cell line, estrogen receptor-positive
Organism Homo sapiens
Characteristics Hormone Starvation: Day 0
Treatment protocol MCF7/BUS cells were inoculated in a plastic dish in DMEM (phenol red-free, 5% stripped fetal calf serum) and cultured for the indicated period (0, 1, or 2 days).
Extracted molecule total RNA
Extraction protocol QIAGEN RNeasy extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
Description Gene expression data from MCF7 (BUS stock) hormone starved for 0 day.
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
 
Submission date Apr 13, 2006
Last update date Apr 13, 2006
Contact name Kathryn Coser
E-mail(s) kcoser@partners.org
Organization name Mass General Hospital Cancer Center
Department DNA Microarray Core
Lab Shioda
Street address Blg 149 13th St
City Charlestown
State/province MA
ZIP/Postal code 02129
Country USA
 
Platform ID GPL96
Series (1)
GSE4668 Expression data from hormone-starved MCF7/BUS cell culture

Data table header descriptions
ID_REF
VALUE MAS5-calculated Signal Intensity

Data table
ID_REF VALUE
AFFX-BioB-5_at 668.5
AFFX-BioB-M_at 1061.3
AFFX-BioB-3_at 739.4
AFFX-BioC-5_at 1694.4
AFFX-BioC-3_at 1183.9
AFFX-BioDn-5_at 1564.8
AFFX-BioDn-3_at 9486.9
AFFX-CreX-5_at 16761.6
AFFX-CreX-3_at 23744.7
AFFX-DapX-5_at 6.8
AFFX-DapX-M_at 66.7
AFFX-DapX-3_at 4.6
AFFX-LysX-5_at 3.2
AFFX-LysX-M_at 68.9
AFFX-LysX-3_at 42.1
AFFX-PheX-5_at 3.6
AFFX-PheX-M_at 5.6
AFFX-PheX-3_at 47.6
AFFX-ThrX-5_at 9.6
AFFX-ThrX-M_at 40.3

Total number of rows: 22283

Table truncated, full table size 357 Kbytes.




Supplementary data files not provided

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap