|
| Status |
Public on Apr 05, 2013 |
| Title |
Drosophila heads_elavII>rCAG.rCUG100_independent line S2, triplicate repeat 2 |
| Sample type |
RNA |
| |
|
| Source name |
Heads, newly eclosed, male, rCAG.rCUG100, S2
|
| Organism |
Drosophila melanogaster |
| Characteristics |
genotype/variation: elavII>rCAG.rCUG100
line: S2
developmental stage: newly eclosed
gender: male
tissue: head
|
| Treatment protocol |
Samples were frozen in liquid nitrogen and homogenised in Trizol reagent (Invitrogen).
|
| Growth protocol |
All the Drosophila stocks and flies were maintained in vials containing Fortified (FI) medium and kept at 25°C. The FI medium was composed of 1% agar, 18.75% compressed yeast, 10% polenta, 10% treacle, 1.5% acid mix and 2.5% tegosept.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using Trizol (Invitrogen), according to the manufacturer's protocol. Total RNA was further purified using the RNeasy Mini Kit (Qiagen).
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared using the One-Cycle Target Labelling and Control Reagents Kit, according to the manufacturer's protocol from 2ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
10ug of fragmented biotinylated cRNA was hybridised for 16hrs at 45oC on a Drosophila Genome 2.0 GeneChip. After 16hrs, GeneChips were washed on a FS_450 Fluidics Station using the Midi_euk_2 v3 script.
|
| Scan protocol |
Data was acquired on a 7G GeneChip Scanner 3000 and data extraction performed in GCOS v1.2.
|
| Description |
Sample 8
|
| Data processing |
CHP files were generated in GCOS 1.2 and normalised by global scaling (MAS5) to a value of 500. Experimental and control lines were compared by both a student t-test and log2 ratios.
|
| |
|
| Submission date |
Dec 13, 2012 |
| Last update date |
Apr 05, 2013 |
| Contact name |
Gareth Price |
| E-mail(s) |
nscalehome@gmail.com
|
| Phone |
61413289611
|
| Organization name |
Queensland Facility for Advanced Bioinformatics
|
| Street address |
Carmody Road
|
| City |
Brisbane |
| State/province |
Queensland |
| ZIP/Postal code |
4072 |
| Country |
Australia |
| |
|
| Platform ID |
GPL1322 |
| Series (1) |
| GSE42910 |
Distinct roles for Toll and autophagy pathways in double-stranded RNA toxicity in a Drosophila model of expanded repeat neurodegenerative diseases |
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