|
Status |
Public on Jan 22, 2013 |
Title |
peripheral blood T-cells Control10 [mRNA] |
Sample type |
RNA |
|
|
Source name |
peripheral blood T-cell
|
Organism |
Homo sapiens |
Characteristics |
group: Control
|
Treatment protocol |
Cells were snap frozen in -80 C
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was prepared using Chomczynski's method (Anal Biochem 162, 156-9 (1987))
|
Label |
Biotin
|
Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 3 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
|
|
Hybridization protocol |
Following fragmentation,15 ug of cRNA were hybridized for 16 hr at 45C on Human U133 Plus 2.0 arrays. Arrays were washed and stained in the Affymetrix Fluidics Station 450.
|
Scan protocol |
After hybridisation (according to the Minimum Information About a Microarray Experiment guideline, the arrays were scanned using an Affymetrix confocal laser scanner (Affymetrix, GeneArray scanner GCS3000) and visualised using GeneChip Operating Software (GCOS, Affymetrix).
|
Description |
gene expression data from T-cell
|
Data processing |
The raw expression signals were preprocessed following the method of Probe Logarithmic Intensity Error (PLIER) based on perfect match only (pm-only) calculation (Affymetrix).The robust quantile method was applied to get normalized expression values.
|
|
|
Submission date |
Dec 28, 2012 |
Last update date |
Sep 01, 2016 |
Contact name |
Intawat Nookaew |
E-mail(s) |
inookaew@uams.edu
|
Organization name |
University of Arkansas for Medical Sciences
|
Department |
Department of Biomedical Informatics
|
Street address |
4301 W Markham St
|
City |
Little Rock |
ZIP/Postal code |
72205 |
Country |
USA |
|
|
Platform ID |
GPL570 |
Series (2) |
GSE43177 |
MicroRNA regulate immunological pathways in T-cells in immune thrombocytopenia (ITP) [mRNA] |
GSE43179 |
MicroRNA regulate immunological pathways in T-cells in immune thrombocytopenia (ITP) |
|
Relations |
Reanalyzed by |
GSE86362 |