|
| Status |
Public on Dec 02, 2013 |
| Title |
HUG1N shRNA Rep2 |
| Sample type |
RNA |
| |
|
| Source name |
HUG1N shRNA treated cells
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: HuG-1
|
| Treatment protocol |
Cells were infected with GATA6 shRNA, selected with puromycin and harvested.
|
| Growth protocol |
ATCC recommended media conditions at 37 degrees.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Labeling was performed following the Affymetrix GeneChip 3’ IVT Express Kit instructions.
|
| |
|
| Hybridization protocol |
Hybridization was performed following the Affymetrix GeneChip 3’ IVT Express Kit instructions; washing and staining was done with the Affymetrix Fluidics Station 450.
|
| Scan protocol |
Scanning was performed according to manufacturer's instructions using the Affymetrix GeneChip Scanner 3000 7G.
|
| Description |
Gene expression data from HuG-1 cells treated with GATA6 shRNA.
|
| Data processing |
The .CEL files were GCRMA normalized using R/Bioconductor.
|
| |
|
| Submission date |
Dec 28, 2012 |
| Last update date |
Dec 02, 2013 |
| Contact name |
Ramesh Shivdasani |
| E-mail(s) |
ramesh_shivdasani@dfci.harvard.edu
|
| Phone |
+1 617 632 5746
|
| Organization name |
Dana-Farber Cancer Institute
|
| Department |
Department of Medical Oncology
|
| Lab |
Ramesh Shivdasani
|
| Street address |
Dana Bldg, Room 720, 450 Brookline Ave
|
| City |
Boston |
| State/province |
Massachusetts |
| ZIP/Postal code |
02115 |
| Country |
USA |
| |
|
| Platform ID |
GPL571 |
| Series (1) |
| GSE43196 |
Genome-wide analyses of GATA6 occupancy and functions provide insights into its oncogenic mechanisms in human gastric cancer (microarray) |
|
