|
| Status |
Public on Feb 03, 2013 |
| Title |
GFP+ B cell blasts - 2 |
| Sample type |
RNA |
| |
|
| Source name |
pre-plasmablasts
|
| Organism |
Mus musculus |
| Characteristics |
background strain: C57BL/6
cell type: pre-plasmablasts
cell population: GFP+
genotype/variation: prdm1-/-
|
| Treatment protocol |
FACS sorted on day 4 of culture based on GFP expression
|
| Growth protocol |
CD40ligand, IL4 (10U/mL) and IL5 (10U/mL)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
| Label |
Cy3
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Data processing |
The data were variance stabilised using lumiT and then quantile normalised using lumi package
|
| |
|
| Submission date |
Dec 31, 2012 |
| Last update date |
Feb 03, 2013 |
| Contact name |
David M Tarlinton |
| E-mail(s) |
tarlinton@wehi.edu.au
|
| Phone |
+61393452555
|
| Fax |
+61393470852
|
| Organization name |
WEHI
|
| Street address |
1G Royal Pde
|
| City |
Parkville |
| State/province |
Vic |
| ZIP/Postal code |
3052 |
| Country |
Australia |
| |
|
| Platform ID |
GPL6333 |
| Series (2) |
| GSE43213 |
Gene expression analysis of B cell differentiation using B cell blasts and developmentally arrested plasmablasts |
| GSE43215 |
Gene expression changes during mouse plasma cell differentiation |
|
