|
| Status |
Public on Apr 25, 2006 |
| Title |
JB1_crgPclp1_MMara_2h_1 |
| Sample type |
RNA |
| |
|
| Source name |
U. maydis strain JB1-crgP::clp1 grown for 2 h in ArrayMedium/Arabinose
|
| Organism |
Ustilago maydis |
| Characteristics |
Strain JB1 is a FB1 derivative with a deletion of the b-mating type (hygromycin replacement). The clp1 gene under control of the arabinose inducable crg1 promoter was introduced in multiple copies into the cbx locus
|
| Biomaterial provider |
Mario Scherer and Jörg Kämper
|
| Treatment protocol |
none
|
| Growth protocol |
JB1-crgP::clp1 was grown to an OD600 of 0.5 at 28°C in liquid array medium: 6.25 % (w/v) Holiday salt solution, 30 mM L-glutamine, 1 % (w/v) glucose, pH 7.0 (filter-sterilized). Subsequently, cells were shifted to liquid array medium containing 1 % (w/v) arabinose instead of glucose as a carbon source, and incubated for 2h.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using the TRIZOL-reagent (Invitrogen) according to the manufacturer’s instructions. RNA samples were further column purified (Qiagen RNeasy) and the quality checked using a Bioanalyzer with a RNA 6000 Nano LabChip kit (Agilent).
|
| Label |
biotin/phycoerythrin
|
| Label protocol |
Target preparation was performed according to the standard Affymetrix protocol using the Enzo BioArray RNA transcript labeling kit, except for a reaction temperature of 50°C for first-strand cDNA synthesis.
|
| |
|
| Hybridization protocol |
Affymetrix EukGE-WS2 on a GeneChip Fluidics Station 400
|
| Scan protocol |
Affymetrix GCS2500
|
| Description |
Experiment to measure the effects of induced expression of the U. maydis clp1 protein. Clp1 is required for the formation of clamp cells during the biotrophic phase of U. maydis.
|
| Data processing |
MAS5.0
|
| |
|
| Submission date |
Apr 19, 2006 |
| Last update date |
Apr 24, 2006 |
| Contact name |
Jörg Thomas Kämper |
| E-mail(s) |
joerg.kaemper@kit.edu
|
| Phone |
+49 721 608 5670
|
| Organization name |
Karlsruhe Institute of Technology
|
| Department |
Department for Applied Biosciences
|
| Street address |
Hertzstrasse 16, Geb. 06.40
|
| City |
Karlsruhe |
| ZIP/Postal code |
76187 |
| Country |
Germany |
| |
|
| Platform ID |
GPL3681 |
| Series (1) |
|
