|
| Status |
Public on Jun 20, 2006 |
| Title |
Su(Hw)-GFP_IP-EmbryoChromatin2a |
| Sample type |
genomic |
| |
|
| Channel 1 |
| Source name |
Control Sera IP
|
| Organism |
Drosophila melanogaster |
| Characteristics |
Chromatin from 0-20 hr Drosophila melanogaster embryos carrying GFP-tagged Su(Hw). Genotype- y[2]; P{su(Hw).GFP}; Df(3R)su(Hw)V, P{casX/K-RpII15} (Gerasimova, TI, Byrd, K and VG Corces (2000) Mol. Cell. 6:p1025) Immunopurified with normal rabbit serum.
|
| Growth protocol |
Egg laying at 25oC
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Chromatin purified as described in Birch-Machin et al (2005) Genome Biology 6:R63, detailed protocol from http://www.flychip.org.uk/protocols/chip/
|
| Label |
Cy5
|
| Label protocol |
Enriched chromatin labelling as described in Birch-Machin et al (2005) Genome Biology 6:R63, detailed protocol from http://www.flychip.org.uk/protocols/chip/
|
| |
|
| Channel 2 |
| Source name |
anti-GFP
|
| Organism |
Drosophila melanogaster |
| Characteristics |
Chromatin from 0-20 hr Drosophila melanogaster embryos carrying GFP-tagged Su(Hw). Genotype- y[2]; P{su(Hw).GFP}; Df(3R)su(Hw)V, P{casX/K-RpII15} (Gerasimova, TI, Byrd, K and VG Corces (2000) Mol. Cell. 6:p1025) Immunopurified with anti-GFP.
|
| Growth protocol |
Egg laying at 25oC
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Chromatin purified as described in Birch-Machin et al (2005) Genome Biology 6:R63, detailed protocol from http://www.flychip.org.uk/protocols/chip/
|
| Label |
Cy3
|
| Label protocol |
Enriched chromatin labelling as described in Birch-Machin et al (2005) Genome Biology 6:R63, detailed protocol from http://www.flychip.org.uk/protocols/chip/
|
| |
|
| |
| Hybridization protocol |
As described in Birch-Machin et al (2005) Genome Biology 6:R63, detailed protocol from http://www.flychip.org.uk/protocols/chip/
|
| Scan protocol |
As described in Birch-Machin et al (2005) Genome Biology 6:R63, detailed protocol from http://www.flychip.org.uk/protocols/chip
|
| Description |
Identification of in vivo Su(Hw) binding sites in Drosophila embryos.
|
| Data processing |
VSN normalisation- VALUE is the VSN normalised ratio of Su(hw) enriched to control. All other data are raw un-normalised values.
|
| |
|
| Submission date |
Apr 20, 2006 |
| Last update date |
May 05, 2006 |
| Contact name |
Steve Russell |
| E-mail(s) |
s.russell@gen.cam.ac.uk
|
| Phone |
+44 (0) 1223 766929
|
| Fax |
+44 (0) 1223 333992
|
| Organization name |
University of Cambridge
|
| Department |
Department of Genetics
|
| Street address |
Downing Street
|
| City |
Cambridge |
| ZIP/Postal code |
CB2 3EH |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL3689 |
| Series (1) |
| GSE4691 |
in vivo binding of su(Hw) in the Drosophila genome |
|
| Data table header descriptions |
| ID_REF |
|
| VALUE |
VSN normalised ratio of anti-sh(Hw) to control sera |
| fgMedian1 |
foreground (spot) median pixel intensity of channel 1 |
| bgMedian1 |
background (local area around spot) median pixel intensity of channel 1 |
| fgAdjMAD1 |
foreground (spot) pixel intensity variability of channel 1 |
| bgAdjMAD1 |
background (local area around spot) pixel intensity variability of channel 1 |
| fgMedian2 |
foreground (spot) median pixel intensity of channel 2 |
| bgMedian2 |
background (local area around spot) median pixel intensity of channel 2 |
| fgAdjMAD2 |
foreground (spot) pixel intensity variability of channel 2 |
| bgAdjMAD2 |
background (local area around spot) pixel intensity variability of channel 2 |
| fgN |
number of pixels in the foreground |
| bgN |
number of pixels in the background |
