|
| Status |
Public on Jan 01, 2016 |
| Title |
Triple (SB/Tg/k5Cre) T257 |
| Sample type |
RNA |
| |
|
| Source name |
Mouse Tissue
|
| Organism |
Mus musculus |
| Characteristics |
tissue: breast
genotype: Triple (SB/Tg/k5Cre)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted by Qiagen RNEasy kit according to the manufacturer's instructions.
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix 3' IVT Express protocol from 100 ng total RNA.
|
| |
|
| Hybridization protocol |
Following fragmentation, cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix 3000 7G Scanner.
|
| Description |
Gene expression data from mouse tissue
|
| Data processing |
Data were normalized by Robust Multichip Average (RMA) using R with Bioconductor.
|
| |
|
| Submission date |
Jan 03, 2013 |
| Last update date |
Jan 01, 2016 |
| Contact name |
Tuan Zea Tan |
| E-mail(s) |
csittz@nus.edu.sg
|
| Organization name |
National University of Singapore
|
| Department |
Cancer Science Institute Singapore
|
| Street address |
14 Medical Drive, #11-01, Singapore
|
| City |
Singapore |
| ZIP/Postal code |
117599 |
| Country |
Singapore |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE43263 |
Sleeping Beauty Expression data from mouse |
|
