|
| Status |
Public on Apr 01, 2013 |
| Title |
Patient 2, Atheroma plaque |
| Sample type |
RNA |
| |
|
| Source name |
Carotid atheroma plaque obtained from endarterectomy
|
| Organism |
Homo sapiens |
| Characteristics |
patient: 2
disease state: hypertension
tissue: Atheroma plaque
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNAs were prepared by IGBMC Microarray and Sequencing Platform according to the standard Affymetrix protocol from 1 ug total RNA.
|
| |
|
| Hybridization protocol |
cRNAs were hybridized for 16 hours at 45oC on GeneChip® Human Gene 1.0 ST arrays using the GeneChip® Hybridization Oven 640 (Affymetrix).
|
| Scan protocol |
The GeneChips were washed and stained in the GeneChip® Fluidics Station 450 (Affymetrix) and scanned using the GeneChip® Scanner 3000 7G System (Affymetrix).
|
| Description |
Gene expression data from paired samples of carotid atheroma plaque and macroscopically intact tissue
JLI_4
|
| Data processing |
The data were analyzed with Expression Console v1.1 software using the default RMA summarization method.
|
| |
|
| Submission date |
Jan 04, 2013 |
| Last update date |
Apr 01, 2013 |
| Contact name |
Catherine Cerutti |
| E-mail(s) |
cerutti@univ-lyon1.fr
|
| Organization name |
Université Lyon 1 & Hôpital Nord-Ouest
|
| Lab |
EA4173 Génomique Fonctionnelle de l'Hypertension Artérielle
|
| Street address |
8 avenue Rockefeller
|
| City |
Lyon |
| ZIP/Postal code |
69373 |
| Country |
France |
| |
|
| Platform ID |
GPL6244 |
| Series (1) |
| GSE43292 |
Genome-wide expression study of human carotid atheroma |
|
