|
Status |
Public on Apr 01, 2013 |
Title |
Patient 13, Atheroma plaque |
Sample type |
RNA |
|
|
Source name |
Carotid atheroma plaque obtained from endarterectomy
|
Organism |
Homo sapiens |
Characteristics |
patient: 13 disease state: hypertension tissue: Atheroma plaque
|
Extracted molecule |
total RNA |
Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
Label |
biotin
|
Label protocol |
Biotinylated cRNAs were prepared by IGBMC Microarray and Sequencing Platform according to the standard Affymetrix protocol from 1 ug total RNA.
|
|
|
Hybridization protocol |
cRNAs were hybridized for 16 hours at 45oC on GeneChip® Human Gene 1.0 ST arrays using the GeneChip® Hybridization Oven 640 (Affymetrix).
|
Scan protocol |
The GeneChips were washed and stained in the GeneChip® Fluidics Station 450 (Affymetrix) and scanned using the GeneChip® Scanner 3000 7G System (Affymetrix).
|
Description |
Gene expression data from paired samples of carotid atheroma plaque and macroscopically intact tissue JLI_44
|
Data processing |
The data were analyzed with Expression Console v1.1 software using the default RMA summarization method.
|
|
|
Submission date |
Jan 04, 2013 |
Last update date |
Apr 01, 2013 |
Contact name |
Catherine Cerutti |
E-mail(s) |
cerutti@univ-lyon1.fr
|
Organization name |
Université Lyon 1 & Hôpital Nord-Ouest
|
Lab |
EA4173 Génomique Fonctionnelle de l'Hypertension Artérielle
|
Street address |
8 avenue Rockefeller
|
City |
Lyon |
ZIP/Postal code |
69373 |
Country |
France |
|
|
Platform ID |
GPL6244 |
Series (1) |
GSE43292 |
Genome-wide expression study of human carotid atheroma |
|