|
| Status |
Public on Aug 01, 2013 |
| Title |
Array 1_3: SUS_3 cy5 + OP_3 cy3 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
five L2 larvae
|
| Organism |
Spodoptera frugiperda |
| Characteristics |
clone: SUS
|
| Treatment protocol |
OP and PYR strains were maintained in the laboratory under selection with chlorpyrifos (at increasing discriminating doses from 100 up to 400 µg of insecticide/g of insect in a topical bioassay) or lambda-cyhalothrin (from 8.4 up to 27 µg of insecticide/g of insect) respectively
|
| Growth protocol |
All strains were maintained on artificial diet at 25± 1°C with a 16:8 L:D photoperiod
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using Trizol following manufacturer's instructions. Genomic DNA was removed by DNase I digestion using the DNA-free DNase Treatment and Removal Reagent (Ambion).
|
| Label |
cy5
|
| Label protocol |
Two micrograms of each RNA sample was used to generate labeled cRNA, this was prepared, hybridized onto arrays and these were washed and scanned exactly as described in the Agilent Quick Amp Labeling Protocol (Version 5.7) which can be downloaded from http://www.chem.agilent.com/en-US/products/instruments/dnamicroarrays/pages/gp11617.aspx.
|
| |
|
| Channel 2 |
| Source name |
five L2 larvae
|
| Organism |
Spodoptera frugiperda |
| Characteristics |
clone: OP
|
| Treatment protocol |
OP and PYR strains were maintained in the laboratory under selection with chlorpyrifos (at increasing discriminating doses from 100 up to 400 µg of insecticide/g of insect in a topical bioassay) or lambda-cyhalothrin (from 8.4 up to 27 µg of insecticide/g of insect) respectively
|
| Growth protocol |
All strains were maintained on artificial diet at 25± 1°C with a 16:8 L:D photoperiod
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using Trizol following manufacturer's instructions. Genomic DNA was removed by DNase I digestion using the DNA-free DNase Treatment and Removal Reagent (Ambion).
|
| Label |
cy3
|
| Label protocol |
Two micrograms of each RNA sample was used to generate labeled cRNA, this was prepared, hybridized onto arrays and these were washed and scanned exactly as described in the Agilent Quick Amp Labeling Protocol (Version 5.7) which can be downloaded from http://www.chem.agilent.com/en-US/products/instruments/dnamicroarrays/pages/gp11617.aspx.
|
| |
|
| |
| Hybridization protocol |
See above
|
| Scan protocol |
Scanned on an Agilent G2565AA scanner.
Images were quantified using Agilent Feature Extraction Software (version 10.5).
|
| Data processing |
Agilent Feature Extraction Software (v 10.5) was used for background subtraction and LOWESS normalization.
|
| |
|
| Submission date |
Jan 04, 2013 |
| Last update date |
Aug 01, 2013 |
| Contact name |
Chris Bass |
| E-mail(s) |
chris.bass@rothamsted.ac.uk
|
| Phone |
01582763133
|
| Organization name |
Rothamsted Research
|
| Department |
Biological Chemistry
|
| Street address |
West Common
|
| City |
Harpenden |
| State/province |
Hertfordshire |
| ZIP/Postal code |
AL52JQ |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL16455 |
| Series (1) |
| GSE43295 |
Comparison of gene expression in three strains of Spodoptera Frugiperda (Insecta: Lepidoptera: Noctuidae) |
|
