|
| Status |
Public on Jan 10, 2013 |
| Title |
B10.BALBChr16 |
| Sample type |
genomic |
| |
|
| Source name |
B10.BALBChr16 Consomic
|
| Organism |
Mus musculus |
| Characteristics |
consomic line: B10.BALBChr16
parental strain: B10.BR
tissue: liver
|
| Treatment protocol |
None
|
| Growth protocol |
DNA was extracted from liver of consomic mice maintained in our breeding colony at the University of Utah.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
DNA was extracted from proteinase-K digested liver DNA using DNeasy Tissue Kit (Qiagen).
|
| Label |
Biotin
|
| Label protocol |
As per manufacturer (Affymetrix)
|
| |
|
| Hybridization protocol |
DNA was restriction digested, PCR amplified, fragmented, labeled and hybridized to each array according to the manufacturer's instructions.
|
| Scan protocol |
The arrays were scanned using an Affymetrix GeneChip Scanner 3000.
|
| Description |
Hybridized to Mouse Diversity Genotyping Array
|
| Data processing |
CEL files were analyzed using R MouseDivGeno software by the JAX Mouse Diversity Genotyping Array Service (Jackson Laboratory).
|
| |
|
| Submission date |
Jan 09, 2013 |
| Last update date |
Jan 10, 2013 |
| Contact name |
Thai Cao |
| E-mail(s) |
thai.cao@hsc.utah.edu
|
| Organization name |
University of Utah
|
| Street address |
30 North 1900 East, SOM5C402
|
| City |
Salt Lake City |
| State/province |
UT |
| ZIP/Postal code |
84108 |
| Country |
USA |
| |
|
| Platform ID |
GPL13147 |
| Series (1) |
| GSE43377 |
Mouse Diversity Array SNP Genotyping of B10.BALBChr16 and BALB.B10Chr16 Reciprocal Consomic Strain Mice |
|
