another strain name: H14msrR*graR*rifR-fdh2* background strain: deltaIP genotype: vraS, msrR, graR mutation (replacement), rpoB mutant (selection with 1 mg/liter rifampin), fdh2 mutation (replacement) growth: up to OD1.0
Extracted molecule
total RNA
Extraction protocol
See reference: Neoh HM, et al.: Mutated response regulator graR is responsible for phenotypic conversion of Staphylococcus aureus from heterogeneous vancomycin-intermediate resistance to vancomycin-intermediate resistance. Antimicrob Agents Chemother. 2008 Jan;52(1):45-53.
Label
Cy3
Label protocol
SuperScript Indirect cDNA Labeling System (Invitrogen Life Technologies, Carlsbad, California).
strain: deltaIP genotype: wild growth: up to OD1.0
Extracted molecule
total RNA
Extraction protocol
See reference: Neoh HM, et al.: Mutated response regulator graR is responsible for phenotypic conversion of Staphylococcus aureus from heterogeneous vancomycin-intermediate resistance to vancomycin-intermediate resistance. Antimicrob Agents Chemother. 2008 Jan;52(1):45-53.
Label
Cy5
Label protocol
SuperScript Indirect cDNA Labeling System (Invitrogen Life Technologies, Carlsbad, California).
Hybridization protocol
Labeled samples were hybridized onto the microarray at 50°C for 16-20 hr. After hybridization, the arrays were washed in the following buffers, and dry by spin slide. 2X SSC/0.05% SDS, for 15 min 0.2X SSC/0.05% SDS, for 15 min 0.5X SSC, for 3 min 0.2X SSC, for 3 min
