|
| Status |
Public on Mar 31, 2014 |
| Title |
RN 2d acclimated 2nd biological pool |
| Sample type |
RNA |
| |
|
| Source name |
RN 2d acclimated 2nd biological pool
|
| Organism |
Rattus norvegicus |
| Characteristics |
tissue: cardiac left ventricle
strain: Sabra strain
age: 1mo
|
| Treatment protocol |
Experimental paradigm included heat acclimation at 34°C for 2d (AC2d) or 30d (AC30), deacclimation (DeAC, 24°C, 30d) and reacclimation (ReAC, 34°C, 2d) . Untreated rats were served as controls ( C ). Rats were euthanized using Ketamin/xylasin and hearts were extirpated. For gene expression analyses, the left ventricle of the heart, two pools of 5 animals each, for each experimental phase were used.
|
| Growth protocol |
Rattus norvegicus, albino, sabra strain, 3 weeks old at the onset of the experiments were used.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Tri-reagent extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cDNA were prepared according to the standard Affymetrix protocol from total RNA (~100-200ng/ul, Expression Analysis Technical Manual, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 5.5 ug of cDNA were hybridized for 16 hr at 45C on GeneChip Rat Gene 1.0 ST Array (Platform GPL6247). GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the GeneChip Scanner 3000 7G
|
| Description |
Gene expression
|
| Data processing |
The data were analyzed with GeneChip Command Console Software (AGCC) using Affymetrix default analysis settings
|
| |
|
| Submission date |
Jan 10, 2013 |
| Last update date |
Mar 31, 2014 |
| Contact name |
Michal Horowitz |
| E-mail(s) |
m.horowitz@mail.huji.ac.il
|
| Phone |
972 2 6757588
|
| Fax |
972 2 6439736
|
| Organization name |
The Hebrew University
|
| Lab |
Lab Environmental Physiology
|
| Street address |
Hadassah Med Center
|
| City |
Jerusalem |
| ZIP/Postal code |
91120 |
| Country |
Israel |
| |
|
| Platform ID |
GPL6247 |
| Series (1) |
| GSE43404 |
Heat acclimation memory: Does the de-acclimated transcriptome reveal epigenetic processes of transcriptional regulation? |
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