|
| Status |
Public on Jan 15, 2013 |
| Title |
SSc Skin |
| Sample type |
other |
| |
|
| Source name |
Human skin obtained by involved skin biopsy from patients with diffuse cutaneous SSc.
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: involved skin of SSc
|
| Extracted molecule |
other |
| Extraction protocol |
The microRNA (miRNA) isolation from human skin tissue was performed using miRNeasy FFPE kit (Qiagen).
|
| Label |
SYBR Green
|
| Label protocol |
For PCR array, miRNAs were reverse-transcribed into first strand cDNA using RT2 miRNA First Strand Kit (SABiosciences). A mixture of equal amounts of miRNAs from 3 normal skins, 3 SSc, 3 LSc or 3 keloid were prepared, and miRNA expression profile in each disease in vivo was evaluated using RT2 Profiler PCR Array. The cDNA was mixed with RT2 SYBR Green/ROX qPCR Master Mix and the mixture was added into 96-well RT2 miRNA PCR Array that includes primer pairs for 88 human miRNAs (SABiosciences).
|
| |
|
| Hybridization protocol |
n/a
|
| Scan protocol |
n/a
|
| Description |
Test
|
| Data processing |
The raw threshold cycle (Ct) was normalized using the value of housekeeping gene, U6.
|
| |
|
| Submission date |
Jan 14, 2013 |
| Last update date |
Jan 15, 2013 |
| Contact name |
Katsunari Makino |
| E-mail(s) |
nerizonanerio@yahoo.co.jp
|
| Organization name |
Kumamoto University
|
| Department |
Dermatology and Plastic Surgery
|
| Street address |
1-1-1 Honjo
|
| City |
Kumamoto |
| State/province |
Kumamoto |
| ZIP/Postal code |
860-8556 |
| Country |
Japan |
| |
|
| Platform ID |
GPL15085 |
| Series (1) |
| GSE43469 |
Real-time quantitative PCR analysis of 88 microRNAs involved in human cell differentiation and development in involved skin of systemic scleroderma (SSc), localized scleroderma (LSc) and Keloid. |
|
