|
| Status |
Public on Jan 07, 2014 |
| Title |
HUVEC_F5_6 |
| Sample type |
RNA |
| |
|
| Source name |
freshly isolated umbilical vein endothelial cells
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: freshly isolated umbilical vein endothelial cells
cell culture bank: Cells were isolated in Clinica Universidad de Navarra after obtaining informed consent
isolation method: human umbilical vein endothelial cells were magnetically purified using CD34 antibody and check for EC purity by CD31, CD34 and CD45 FACS analysis. Samples were directly processed for RNA extraction without any kind of culture
|
| Extracted molecule |
total RNA |
| Extraction protocol |
The cells were harvested with TRIzol Reagent (Invitrogen) and the RNA was extracted according to the manufacturer's instructions. As a last step of the extraction procedure, the RNA was purified with the RNeasy MiniKit (Qiagen, Hilden, Germany).
|
| Label |
biotin
|
| Label protocol |
The cDNA was prepared from 2 microg total RNA using the One –Cycle cDNA Synthesis kit from Affymetrix. This cDNA was used for synthezising the cRNA using the IVT labeling kit (Affymetrix). The synthezised cRNA was purified with the GeneChip Sample Cleanup Module (Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 microg of cRNA were hybridized to the Affymetrix HG-U133Plus2 chip according to the protocols provided by the manufacturer.
|
| Scan protocol |
GeneChips were scanned using the GeneChip Scanner 3000 from Affymetrix.
|
| Description |
venous endothelial cells
|
| Data processing |
Data were normalized with Bioconductor software using RMA algorithm.
|
| |
|
| Submission date |
Jan 14, 2013 |
| Last update date |
Jan 07, 2014 |
| Contact name |
Xabier Agirre |
| E-mail(s) |
xaguirre@unav.es
|
| Phone |
+34 948194700
|
| Organization name |
CIMA
|
| Department |
Oncology
|
| Street address |
Pio XII, 55
|
| City |
Pamplona |
| State/province |
Navarra |
| ZIP/Postal code |
31008 |
| Country |
Spain |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE43475 |
UNRAVELING A NOVEL TRANSCRIPTION FACTOR CODE INDUCTIVE FOR THE HUMAN ARTERIAL-SPECIFIC ENDOTHELIAL CELL SIGNATURE |
|
