|
| Status |
Public on Nov 03, 2006 |
| Title |
short term cultured GBM_ID GLI60T2_5`aza-dc_96h_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
short term cultured GBM_ID GLI60T2_5`aza-dc_96h
|
| Organism |
Homo sapiens |
| Characteristics |
short term cultured glioblastoma_GLI60_passage 15
|
| Treatment protocol |
16h after seeding the cells were treated with 5umol 5`aza-dC for 96h.
|
| Growth protocol |
cells were cultured in L-Glutamine free DMEM. Supplement with 1% L-Glutamine, heat inactivated 10%FBS, 1% Penicillin/Streptomycin. Grown in a 37° humidified atmosphere of 5% CO2/95%air.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
TRIzol extraction (Invitrogen Life Technologies) followed by clean-up using RNaesy Mini kit (Quiagen) according to manufacturers instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
short term cultured glioblastoma sample_ID GLI60_treated with 5`aza-dC for 96h_replica 2
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
|
| |
|
| Submission date |
Apr 24, 2006 |
| Last update date |
Nov 03, 2006 |
| Contact name |
Wolf C Mueller |
| E-mail(s) |
wolf_muller@yahoo.de, wolf.mueller@charite.de
|
| Phone |
+49-(0)30-450536004
|
| Fax |
+49-(0)30-450536940
|
| Organization name |
Charité Universitätsmedizin Berlin
|
| Department |
Neuropathology
|
| Street address |
Amrumer Str. 1
|
| City |
Berlin |
| ZIP/Postal code |
13353 |
| Country |
Germany |
| |
|
| Platform ID |
GPL96 |
| Series (1) |
| GSE4717 |
5`aza-dC demethylation of three short term cultured glioblastomas |
|
